基于 LC-PDA-MS/MS 的长柄土楠根中关键植物化学成分的表征。

LC-PDA-MS/MS-Based Characterization of Key Phytochemicals in Eurycoma Longifolia Roots.

机构信息

Institute of Bioproduct Development, Universiti Teknologi Malaysia, 81310 UTM Skudai, Johor Bahru, Johor, Malaysia.

Department of Bioprocess and Polymer Engineering, School of Chemical and Energy Engineering, Faculty of Engineering, Universiti Teknologi Malaysia, 81310 UTM Skudai, Johor Bahru, Johor, Malaysia.

出版信息

J Chromatogr Sci. 2021 Jun 21;59(7):659-669. doi: 10.1093/chromsci/bmab041.

DOI:10.1093/chromsci/bmab041

PMID:33876232

Abstract

The objective of the study was to fractionate the crude extract of Eurycoma longifolia (E. longifolia) roots and identify the intense peaks using HPLC-PDA-MS/MS, UPLC-MS/MS and H-NMR. Column chromatography was used to fractionate the crude extract into individual fractions using six solvent systems ranged from ethyl acetate, methanol and water in increasing polarity. Two fractions with nearly pure and intense peaks were selected for compound identification. Chromenone (coumarin) and chromone derivatives were putatively identified, besides several previously reported quassinoid glycosides (eurycomanone derived glycoside, 2,3-dehydro-4α-hydroxylongilactone glucoside, eurycomanol glycoside and eurycomanol trimer) in the fraction 11 of 100% methanol. A newly reported compound, namely hydroxyl glyyunanprosapogenin D (838 g/mol) was proposed to be the compound detected in the fraction 11 of 50% ethyl acetate and 50% methanol. This is also the first study to report the identification of chromenones and chromones in E. longifolia extract.

摘要

本研究的目的是对长柄钩吻（Eurycoma longifolia）根的粗提取物进行分级，并使用 HPLC-PDA-MS/MS、UPLC-MS/MS 和 H-NMR 鉴定强烈的峰。使用六种溶剂系统（从乙酸乙酯、甲醇和水到极性增加）通过柱层析将粗提取物分离成单个馏分。选择具有近乎纯和强烈峰的两个馏分进行化合物鉴定。推测鉴定出香豆酮（香豆素）和色酮衍生物，此外，在 100%甲醇的馏分 11 中还鉴定出几种先前报道的奎宁oid 糖苷（源自 eurycomanone 的糖苷、2,3-脱氢-4α-羟基龙脑内酯葡萄糖苷、eurycomanol 糖苷和 eurycomanol 三聚体）。在 50%乙酸乙酯和 50%甲醇的馏分 11 中提出了一种新报道的化合物，即羟基 glyyunanprosapogenin D（838g/mol）。这也是首次报道在 E. longifolia 提取物中鉴定出香豆酮和色酮。