Department of Botany, Lahore College for Women University, Lahore, Pakistan.
Department of Botany, Faculty of Life Sciences, University of Okara, Okara, Pakistan.
Microsc Res Tech. 2021 Oct;84(10):2286-2290. doi: 10.1002/jemt.23782. Epub 2021 Apr 20.
Grass taxonomy is quite problematic and insignificant taxonomic work has been done on Echinochloa with special reference to Pakistan. Therefore, the present study was carried out to provide taxonomic keys for the identification of this genus through microscopic epidermal leaf anatomical parameters. Light microscopy and scanning electron microscopy were done to delimit the different species. Special structures like hook cells, short and long cells, stomata, macro- and micro-hairs, prickles and silica bodies showed a lot of variation among each species. For instance, E. frumentacea can be distinguished from E. walteri by the presence of macro hairs between the veins. Furthermore, E. crus-galli can be distinguished from E. frumentacea in terms of macro hair size, that is, 45-60 μm and 20-60 μm, respectively. Similarly, E. colona showed marked variations in terms of prickles abundance from E. frumentacea. Overall results of this research reports valuable qualitative and quantitative diagnostic futures for the genus Echinochloa.
禾本科植物分类学存在较多问题，且针对巴基斯坦地区的稗属植物的分类学工作也很少。因此，本研究通过叶片表皮解剖结构的微观参数为该属植物的鉴定提供分类学依据。利用光学显微镜和扫描电子显微镜对不同种进行了划分。钩细胞、短细胞和长细胞、气孔、大毛和小毛、刺以及硅质体等特殊结构在各物种之间存在明显差异。例如，Echinochloa frumentacea 可通过脉间存在大毛与 E. walteri 相区别。此外，Echinochloa crus-galli 可根据大毛的大小与 E. frumentacea 相区分，分别为 45-60μm 和 20-60μm。同样，Echinochloa colona 在刺的数量上与 E. frumentacea 存在明显差异。本研究的总体结果为稗属植物提供了有价值的定性和定量鉴别特征。
