National Institute of Environmental Health, Chinese Center for Disease Control and Prevention, China CDC Key Laboratory of Environment and Population Health, Beijing, 100021, China.
China State Key Laboratory of Environmental Criteria and Risk Assessment, Chinese Research Academy of Environmental Sciences, Beijing, 100012, China.
Curr Med Sci. 2021 Apr;41(2):189-198. doi: 10.1007/s11596-021-2336-z. Epub 2021 Apr 20.
The etiology for the high tumor mortality in heavy polluted Yinghe river basin is still unclear and polycyclic aromatic hydrocarbons (PAHs) belong to the priority pollutants in water based on the former surveillance data. In order to explore the potential genotoxicants contributing to the double-endpoint genotoxicity of polluted drinking water source, 12 groundwater and 3 surface water samples were collected from 3 villages and the nearby rivers alongside Yinghe river basin, respectively and their comprehensive genotoxicity was estimated with a bioassay group of SOS/umu test and micronucleus (MN) test (MNT). Some groundwater samples showed positive genotoxicity and all surface water samples were highly genotoxic. Eight groundwater samples showed DNA genotoxic effect with the average 4-NQO equivalent concentration (TEQ) of 0.067 µg/L and 0.089 µg/L in wet and dry season, respectively. The average MN ratios of groundwater samples were 14.19‰ and 17.52‰ in wet and dry season, respectively. Groundwater samples showed different genotoxic effect among 3 villages. The total PAHs concentrations in all water samples ranged from 8.98 to 25.17 ng/L with an average of 14.97±4.85 ng/L. BaA, CHR, BkF, BaP and DBA were the main carcinogenic PAHs contributing to the genotoxicity of water samples. In conclusion, carcinogenic PAHs are possibly related to the high tumor mortality in the target area. Characterization of carcinogenic PAHs to genotoxicity of drinking water source may shed light on the etiology study for high tumor mortality in Yinghe river basin.
应河重污染流域肿瘤死亡率高的病因仍不清楚，根据以往监测数据，多环芳烃（PAHs）属于水中优先污染物。为了探讨导致污染饮用水源双重终点遗传毒性的潜在遗传毒性物质，从 3 个村庄和应河流域附近的河流中分别采集了 12 个地下水和 3 个地表水样本，并用 SOS/umu 测试和微核（MN）测试（MNT）的生物测定组评估其综合遗传毒性。一些地下水样本显示出阳性遗传毒性，所有地表水样本均具有高度遗传毒性。8 个地下水样本显示出 DNA 遗传毒性效应，其 4-NQO 当量浓度（TEQ）在湿季和干季的平均值分别为 0.067 µg/L 和 0.089 µg/L。地下水样本在湿季和干季的 MN 比值平均值分别为 14.19‰和 17.52‰。地下水样本在 3 个村庄之间显示出不同的遗传毒性效应。所有水样中的总多环芳烃浓度范围为 8.98 至 25.17 ng/L，平均值为 14.97±4.85 ng/L。BaA、CHR、BkF、BaP 和 DBA 是导致水样遗传毒性的主要致癌多环芳烃。总之，致癌多环芳烃可能与目标区域的高肿瘤死亡率有关。多环芳烃对饮用水源遗传毒性的特征描述可能有助于阐明应河流域高肿瘤死亡率的病因研究。
