Department of Biology and Department of Biomedical and Pharmaceutical Sciences, Kyung Hee University, Seoul, 02447, Republic of Korea.
J Microbiol. 2021 Jun;59(6):552-562. doi: 10.1007/s12275-021-1036-5. Epub 2021 Apr 20.
A polyphasic taxonomic approach was used to characterize three novel bacterial strains, designated as HDW12A, HDW-15B, and HDW15C, isolated from the intestine of fish species Odontobutis interrupta or Siniperca scherzeri. All isolates were obligate aerobic, non-motile bacteria, and grew optimally at 30°C. Phylogenetic analysis based on 16S rRNA sequences revealed that strain HDW12A was a member of the genus Nocardioides, and closely related to Nocardioides allogilvus CFH 30205 (98.9% sequence identities). Furthermore, strains HDW15B and HDW15C were members of the genus Sphingomonas, and closely related to Sphingomonas lutea JS5 and Sphingomonas sediminicola Dae 20 (97.1% and 97.9% sequence identities), respectively. Strain HDW12A contained MK-8 (H), and strains HDW15B and HDW15C contained Q-10 as the respiratory quinone. Major polar lipid components of strain HDW12A were diphosphatidylglycerol, phosphatidylglycerol, and phosphatidylinositol, and those of strains HDW15B and HDW15C were sphingoglycolipid, diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, and phosphatidylcholine. The G + C content of strains HDW12A, HDW15B, and HDW15C were 69.7, 63.3, and 65.5%, respectively. The results of phylogenetic, phenotypic, chemotaxonomic, and genotypic analyses suggest that strain HDW12A represents a novel species within the genus Nocardioides, and strains HDW15B and HDW15C represent two novel species within the genus Sphingomonas. We propose the names Nocardioides piscis for strain HDW12A (= KACC 21336 = KCTC 49321 = JCM 33670), Sphingomonas piscis for strain HDW15B (= KACC 21341 = KCTC 72588 = JCM 33738), and Sphingomonas sinipercae for strain HDW15C (= KACC 21342 = KCTC 72589 = JCM 33739).
采用多相分类法对从鱼类 Odontobutis interrupta 或 Siniperca scherzeri 的肠道中分离到的三株新型细菌菌株 HDW12A、HDW-15B 和 HDW15C 进行了表征。所有分离株均为需氧、非运动细菌，最佳生长温度为 30°C。基于 16S rRNA 序列的系统发育分析表明，菌株 HDW12A 是 Nocardioides 属的成员，与 Nocardioides allogilvus CFH 30205（98.9%序列同一性）密切相关。此外，菌株 HDW15B 和 HDW15C 是 Sphingomonas 属的成员，与 Sphingomonas lutea JS5 和 Sphingomonas sediminicola Dae 20（97.1%和 97.9%序列同一性）密切相关。菌株 HDW12A 含有 MK-8（H），而菌株 HDW15B 和 HDW15C 含有 Q-10 作为呼吸醌。菌株 HDW12A 的主要极性脂成分为双磷脂酰甘油、磷脂酰甘油和磷脂酰肌醇，而菌株 HDW15B 和 HDW15C 的主要极性脂成分为神经酰胺糖脂、双磷脂酰甘油、磷脂酰甘油、磷脂酰乙醇胺和磷脂酰胆碱。菌株 HDW12A、HDW15B 和 HDW15C 的 G+C 含量分别为 69.7%、63.3%和 65.5%。基于系统发育、表型、化学分类和基因型分析的结果表明，菌株 HDW12A 代表 Nocardioides 属中的一个新种，而菌株 HDW15B 和 HDW15C 代表 Sphingomonas 属中的两个新种。我们提议将菌株 HDW12A 命名为 Nocardioides piscis（=KACC 21336=KCTC 49321=JCM 33670），将菌株 HDW15B 命名为 Sphingomonas piscis（=KACC 21341=KCTC 72588=JCM 33738），将菌株 HDW15C 命名为 Sphingomonas sinipercae（=KACC 21342=KCTC 72589=JCM 33739）。
