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sp. nov.,分离自韩国农田土壤。

sp. nov., isolated from farmland soil in the Republic of Korea.

机构信息

Department of Biotechnology, Pukyong National University, Busan 608-737, Republic of Korea.

Industrial Biomaterial Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), 125 Gwahak-ro, Yuseong-gu, Daejeon 34141, Republic of Korea.

出版信息

Int J Syst Evol Microbiol. 2022 Feb;72(2). doi: 10.1099/ijsem.0.004928.

Abstract

A Gram-stain positive, aerobic, irregularly rod-shaped, non-spore-forming bacterium, designated as BN130099, was isolated from farmland soil sampled in Goesan-gun, Chungbuk, Republic of Korea. Phylogenetic analysis of its 16S rRNA gene sequence showed that the strain is closely related to KACC 19192 with 98.11 % similarity. The DNA G+C content of strain BN130099 was 68.84 mol% (draft genome sequence). The genome sequence of BN130099 displayed key enzymes involved in bioremediation of organic pollutants and biosynthetic clusters of saquayamycin. The strain contained ll-2,6-diaminopimelic acid in the cell-wall peptidoglycan and MK-8(H) as the major respiratory quinone. The predominant fatty acid was iso-C. The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine and phosphatidylinositol. The results of physiological and biochemical characterization allowed the phenotypic differentiation of strain BN130099 from KACC 19192. The strain represents a novel species of the genus , for which we propose the name sp. nov. The type strain is BN130099 (=KCTC 49079=CCTCC AB 2018135).

摘要

一株革兰氏阳性、需氧、不规则杆状、不产芽孢的细菌,被命名为 BN130099,从韩国忠清北道戈山郡的农田土壤中分离得到。其 16S rRNA 基因序列的系统发育分析表明,该菌株与 KACC 19192 具有 98.11%的相似性。菌株 BN130099 的 DNA G+C 含量为 68.84 mol%(基因组草图)。BN130099 的基因组序列显示了参与有机污染物生物修复和 saquayamycin 生物合成簇的关键酶。该菌株细胞壁肽聚糖中含有 ll-2,6-二氨基庚二酸,主要呼吸醌为 MK-8(H)。主要脂肪酸为 iso-C。主要极性脂为双磷脂酰甘油、磷脂酰甘油、磷脂酰胆碱和磷脂酰肌醇。生理生化特征的研究结果允许从 KACC 19192 中对菌株 BN130099 进行表型区分。该菌株代表了属的一个新种,我们建议将其命名为 sp. nov. 。模式菌株为 BN130099 (=KCTC 49079=CCTCC AB 2018135)。

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