sp. nov., isolated from the intestine of the dark diving beetle , and sp. nov., isolated from the intestine of the diving beetle .

A polyphasic taxonomic approach was used to characterize two novel bacterial strains, designated as HDW11 and HDW19, isolated from intestine samples of the dark diving beetle and the diving beetle , respectively. Both isolates were Gram-stain-positive, facultatively anaerobic and non-motile. Strain HDW11 grew optimally at 30 °C, pH 8 and in the presence of 1% (w/v) NaCl. Strain HDW19 grew optimally at 25 °C, pH 7 and in the presence of 0.3% (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences and genome sequences revealed that strain HDW11 is a member of the genus and is closely related to VG341 [with 97.9% 16S rRNA sequence identity and 79.1% average nucleotide identity (ANI)], and that strain HDW19 belongs to the genus and is closely related to KCTC 3621 (with 98.9% 16S rRNA sequence identity and 79.5% ANI). The major cellular fatty acids of strains HDW11 and HDW19 were C 9c and anteiso-C, respectively. The sole respiratory quinone of strain HDW11 was MK-9 (H). The major polar lipid components of strain HDW11 were diphosphatidylglycerol and phosphatidylglycerol, and the major polar lipid component of strain HDW19 was diphosphatidylglycerol. The genomic DNA G+C content of strains HDW11 and HDW19 were 72.1 and 37.2 mol%, respectively. The results of phylogenetic, phenotypic, chemotaxonomic and genotypic analyses suggest that strain HDW11 represents a novel species within the genus , and that strain HDW19 represents a novel species within the genus . We propose the name sp. nov. for strain HDW11 (=KACC 21335=KCTC 49320=JCM 33680) and the name for strain HDW19 (=KACC 21347=KCTC 43114=JCM 33684).