Department of Biology and Department of Biomedical and Pharmaceutical Sciences, Kyung Hee University, Seoul 02447, Republic of Korea.
Int J Syst Evol Microbiol. 2021 Apr;71(4). doi: 10.1099/ijsem.0.004779.
A polyphasic taxonomic approach was used to characterize two novel bacterial strains, designated as HDW11 and HDW19, isolated from intestine samples of the dark diving beetle and the diving beetle , respectively. Both isolates were Gram-stain-positive, facultatively anaerobic and non-motile. Strain HDW11 grew optimally at 30 °C, pH 8 and in the presence of 1% (w/v) NaCl. Strain HDW19 grew optimally at 25 °C, pH 7 and in the presence of 0.3% (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences and genome sequences revealed that strain HDW11 is a member of the genus and is closely related to VG341 [with 97.9% 16S rRNA sequence identity and 79.1% average nucleotide identity (ANI)], and that strain HDW19 belongs to the genus and is closely related to KCTC 3621 (with 98.9% 16S rRNA sequence identity and 79.5% ANI). The major cellular fatty acids of strains HDW11 and HDW19 were C 9c and anteiso-C, respectively. The sole respiratory quinone of strain HDW11 was MK-9 (H). The major polar lipid components of strain HDW11 were diphosphatidylglycerol and phosphatidylglycerol, and the major polar lipid component of strain HDW19 was diphosphatidylglycerol. The genomic DNA G+C content of strains HDW11 and HDW19 were 72.1 and 37.2 mol%, respectively. The results of phylogenetic, phenotypic, chemotaxonomic and genotypic analyses suggest that strain HDW11 represents a novel species within the genus , and that strain HDW19 represents a novel species within the genus . We propose the name sp. nov. for strain HDW11 (=KACC 21335=KCTC 49320=JCM 33680) and the name for strain HDW19 (=KACC 21347=KCTC 43114=JCM 33684).
采用多相分类方法对从 和 肠道样本中分离得到的两株新型细菌菌株进行了表征，分别命名为 HDW11 和 HDW19。这两株菌均为革兰氏阳性、兼性厌氧且不运动。菌株 HDW11 在 30°C、pH8 和 1%（w/v）NaCl 存在下生长最佳。菌株 HDW19 在 25°C、pH7 和 0.3%（w/v）NaCl 存在下生长最佳。基于 16S rRNA 基因序列和基因组序列的系统发育分析表明，菌株 HDW11 是 属的成员，与 VG341[具有 97.9%的 16S rRNA 序列同一性和 79.1%的平均核苷酸同一性（ANI）]密切相关，而菌株 HDW19 属于 属，与 KCTC 3621 密切相关（具有 98.9%的 16S rRNA 序列同一性和 79.5%的 ANI）。菌株 HDW11 和 HDW19 的主要细胞脂肪酸分别为 C9c 和 anteiso-C。菌株 HDW11 的唯一呼吸醌为 MK-9（H）。菌株 HDW11 的主要极性脂成分为二磷脂酰甘油和磷脂酰甘油，而菌株 HDW19 的主要极性脂成分为二磷脂酰甘油。菌株 HDW11 和 HDW19 的基因组 DNA G+C 含量分别为 72.1 和 37.2mol%。基于系统发育、表型、化学分类和基因分析的结果表明，菌株 HDW11 代表了 属中的一个新种，而菌株 HDW19 代表了 属中的一个新种。我们建议将菌株 HDW11 命名为 sp. nov.（=KACC 21335=KCTC 49320=JCM 33680），将菌株 HDW19 命名为 （=KACC 21347=KCTC 43114=JCM 33684）。
