Liver Pathology Unit, Departments of Biochemistry and Microbiology, Hospital Universitari Vall d'Hebron, Universitat Autònoma De Barcelona, Barcelona Spain.
Clinical Biochemistry Research Group, Vall d'Hebron Institut Recerca (VHIR), Hospital Universitari Vall d'Hebron, Universitat Autònoma de Barcelona, Barcelona, Spain.
Expert Rev Mol Diagn. 2021 Apr;21(4):381-396. doi: 10.1080/14737159.2021.1913055. Epub 2021 Apr 21.
Hepatitis B virus (HBV) causes a complex and persistent infection with a major impact on patients health. Viral-genome sequencing can provide valuable information for characterizing virus genotype, infection dynamics and drug and vaccine resistance.
This article reviews the current literature to describe the next-generation sequencing progress that facilitated a more comprehensive study of HBV quasispecies in diagnosis and clinical monitoring.
HBV variability plays a key role in liver disease progression and treatment efficacy. Second-generation sequencing improved the sensitivity for detecting and quantifying mutations, mixed genotypes and viral recombination. Third-generation sequencing enables the analysis of the entire HBV genome, although the high error rate limits its use in clinical practice.
乙型肝炎病毒（HBV）可引起复杂且持续的感染，对患者的健康有重大影响。病毒基因组测序可为病毒基因型、感染动态以及药物和疫苗耐药性的特征提供有价值的信息。
本文综述了当前文献，描述了下一代测序技术的进展，这些进展促进了对 HBV 准种在诊断和临床监测中的更全面研究。
HBV 的变异性在肝病进展和治疗效果中起着关键作用。第二代测序提高了检测和定量突变、混合基因型和病毒重组的灵敏度。第三代测序可分析整个 HBV 基因组，但高错误率限制了其在临床实践中的应用。
