Collaborative Innovation Center of Yangtze River Delta Region Green Pharmaceuticals, Zhejiang University of Technology, Hangzhou, 310014, Zhejiang, People's Republic of China.
School of Pharmaceutical Sciences, Zhejiang University of Technology, Hangzhou, 310014, Zhejiang, People's Republic of China.
Microb Cell Fact. 2021 Apr 21;20(1):87. doi: 10.1186/s12934-021-01579-2.
Trans-4-hydroxy-L-proline is an important amino acid that is widely used in medicinal and industrial applications, particularly as a valuable chiral building block for the organic synthesis of pharmaceuticals. Traditionally, trans-4-hydroxy-L-proline is produced by the acidic hydrolysis of collagen, but this process has serious drawbacks, such as low productivity, a complex process and heavy environmental pollution. Presently, trans-4-hydroxy-L-proline is mainly produced via fermentative production by microorganisms. Some recently published advances in metabolic engineering have been used to effectively construct microbial cell factories that have improved the trans-4-hydroxy-L-proline biosynthetic pathway. To probe the potential of microorganisms for trans-4-hydroxy-L-proline production, new strategies and tools must be proposed. In this review, we provide a comprehensive understanding of trans-4-hydroxy-L-proline, including its biosynthetic pathway, proline hydroxylases and production by metabolic engineering, with a focus on improving its production.
反-4-羟脯氨酸是一种重要的氨基酸，在医药和工业应用中被广泛应用，特别是作为手性砌块，用于有机合成药物。传统上，反-4-羟脯氨酸是通过胶原蛋白的酸性水解产生的，但这个过程存在严重的缺点，如生产力低、工艺复杂和严重的环境污染。目前，反-4-羟脯氨酸主要通过微生物发酵生产。一些最近发表的代谢工程方面的进展已经被用于有效地构建微生物细胞工厂，从而改进了反-4-羟脯氨酸的生物合成途径。为了探究微生物生产反-4-羟脯氨酸的潜力，必须提出新的策略和工具。在这篇综述中，我们全面地介绍了反-4-羟脯氨酸，包括其生物合成途径、脯氨酸羟化酶和代谢工程生产，重点是提高其产量。
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