Department of Chemical Engineering, Faculty of Engineering, Graduate School, Kyushu University, 744 Motooka, Nishi-ku, Fukuoka 819-0395, Japan.
Global Innovation Center, Kyushu University, Fukuoka Industry-Academia Symphonicity, 4-1 Kyudai-Shinmachi, Nishi-ku, Fukuoka-city, Fukuoka 819-0388, Japan.
J Biosci Bioeng. 2021 Jul;132(1):95-101. doi: 10.1016/j.jbiosc.2021.03.015. Epub 2021 Apr 18.
Growth factors (GFs) are indispensable in regenerative medicine because of their high effectiveness. However, as GFs degenerate easily, the development of a suitable carrier with improved stability for GFs is necessary. In this study, we developed a gel-in-oil (G/O) emulsion technology for the transdermal delivery of growth factors. Nanogel particles prepared with heparin-immobilized gelatin that can bind growth factors were dispersed in isopropyl myristate. The particle size of the G/O emulsion could be controlled by changing the surfactant concentration, volume ratio of the water phase to the oil phase, and gelatin concentration. In vitro skin penetration studies showed better penetration through the stratum corneum of fluorescent proteins containing G/O emulsions than of the aqueous solution of GF. Similarly, an in vivo study showed an angiogenesis-inducing effect after transdermal application of GF-immobilized G/O emulsion. Angiogenesis in mice was confirmed owing to both an increased blood vessel network and higher hemoglobin content in the blood. Therefore, the G/O emulsion could be a promising carrier for GFs with better stability and can effectively deliver GFs at the target site.
生长因子（GFs）在再生医学中不可或缺，因为它们具有很高的功效。然而，由于 GFs 容易退化，因此需要开发一种具有改善的稳定性的合适载体来携带 GFs。在本研究中，我们开发了一种用于生长因子经皮传递的油包凝胶（G/O）乳液技术。用肝素固定化明胶制备纳米凝胶颗粒，可与生长因子结合，分散在肉豆蔻异丙酯中。通过改变表面活性剂浓度、水相和油相的体积比以及明胶浓度，可以控制 G/O 乳液的粒径。体外皮肤渗透研究表明，含有 G/O 乳液的荧光蛋白比 GF 的水溶液具有更好的角质层穿透能力。同样，经皮应用 GF 固定化 G/O 乳液后的体内研究表明具有诱导血管生成的作用。由于血管网络增加和血液中血红蛋白含量升高，在小鼠中证实了血管生成。因此，G/O 乳液可能是一种具有更好稳定性的生长因子的有前途的载体，并可以有效地将生长因子递送到靶位。
