Sharifan Amin, Etebari Mahmoud, Zolfaghari Behzad, Aliomrani Mehdi
Department of Pharmacology, Isfahan Pharmaceutical Sciences Research Center, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran.
Department of Pharmacognosy, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran.
Toxicol Res (Camb). 2021 Mar 23;10(2):223-231. doi: 10.1093/toxres/tfab006. eCollection 2021 Mar.
Cisplatin is used for treating multiple types of cancers. Alongside its therapeutic effects, there are side effects, including cytotoxicity and genotoxicity for healthy cells, which are mainly related to radical oxygen species (ROS) production by the drug. These side effects could troublesome the treatment process. Previous studies have suggested that members of Pinaceae family are rich sources of antioxidant components. This article investigates the antioxidant activity (AA) of (Pinaceae) along with its cyto/genoprotective effects following cisplatin exposure on human umbilical vein endothelial cells (HUVECs) cell line. 's hydroalcoholic bark extract (PEHABE) and 's needle volatile oil (PENVO) were prepared using maceration and hydrodistillation methods, respectively. PENVO was analysed via gas chromatograph-mass spectrometry, and the total phenolic content of PEHBAE was measured by folin-ciocalteu reagent. AA of both PEHABE and PENVO were determined using DPPH assay. Moreover, MTT test was used to determine the cytoprotective effects of both agents. Comet and micronucleus (MN) tests were also performed to investigate the genoprotective effect of . Germacrene D (35.72%) was the main component of PENVO. PEHABE showed higher AA compared with PENVO, with the highest AA observed at 25 and 250 μg/ml, respectively. Both PENVO and PEHABE were cytoprotective, with the latter having mitogenic effects on cells at 75, 100, and 200 μg/ml concentrations ( < 0.01 and < 0.001). Also, both PEHABE and PENVO showed genoprotective effects against cisplatin in comet assay ( < 0.001). As PEHABE's concentrations were increased, a reduced number of MN formation was observed after cisplatin's exposure ( < 0.001). In conclusion, PEHABE had higher AA compared with PENVO, and both agents had cyto/genoprotective effects on HUVECs.
顺铂用于治疗多种类型的癌症。除了其治疗效果外，还存在副作用，包括对健康细胞的细胞毒性和遗传毒性，这主要与药物产生的活性氧（ROS）有关。这些副作用可能会给治疗过程带来麻烦。先前的研究表明，松科植物富含抗氧化成分。本文研究了松科植物提取物对人脐静脉内皮细胞（HUVECs）细胞系的抗氧化活性（AA）及其在顺铂暴露后的细胞/基因保护作用。分别采用浸渍法和水蒸馏法制备了该松科植物的树皮水醇提取物（PEHABE）和针叶挥发油（PENVO）。通过气相色谱-质谱联用仪分析PENVO，并采用福林-酚试剂测定PEHBAE的总酚含量。使用DPPH法测定PEHABE和PENVO的AA。此外，采用MTT试验测定两种药物的细胞保护作用。还进行了彗星试验和微核（MN）试验以研究该松科植物提取物的基因保护作用。吉马烯D（35.72%）是PENVO的主要成分。与PENVO相比，PEHABE表现出更高的AA，分别在25和250μg/ml时观察到最高的AA。PENVO和PEHABE均具有细胞保护作用，后者在75、100和200μg/ml浓度下对细胞具有促有丝分裂作用（P<0.01和P<0.001）。此外，在彗星试验中，PEHABE和PENVO均对顺铂表现出基因保护作用（P<0.001）。随着PEHABE浓度的增加，顺铂暴露后观察到的MN形成数量减少（P<0.001）。总之，与PENVO相比，PEHABE具有更高的AA，且两种药物对HUVECs均具有细胞/基因保护作用。
