Ikewuchi Catherine C, Ifeanacho Mercy O, Ikewuchi Jude C
Department of Biochemistry, Faculty of Science.
Department of Food Science, Faculty of Agriculture, University of Port Harcourt, P.M.B. 5323, Port Harcourt, Nigeria.
Porto Biomed J. 2021 Feb 11;6(1):e129. doi: 10.1097/j.pbj.0000000000000129. eCollection 2021 Jan-Feb.
The major draw-back of doxorubicin's use in chemotherapy is its toxicity on various organs including the kidneys. This study investigated the potential protective role of aqueous leaf-extracts of and against nephrotoxicity induced by doxorubicin.
To this end, their impact on plasma biomarkers of kidney function, as well as renal lipid profile, biomarkers of oxidative stress, electrolyte profile and activities of renal ATPases was monitored in doxorubicin treated rats. Metformin (250 mg/kg body weight, orally) and the extracts (50, 75 and 100 mg/kg, orally) were daily administered for 14 days; while nephrotoxicity was induced with doxorubicin (15 mg/kg, intra-peritioneally), once on the 12th day of study.
The plasma concentrations of creatinine, and urea; as well as the renal malondialdehyde, cholesterol, calcium and sodium concentrations in the Test control, were significantly ( < .05) higher than those of all the other groups. However, the renal concentrations of ascorbic acid, chloride, magnesium and potassium, and the renal activities of catalase, glutathione peroxidase superoxide dismutase, Ca-ATPase, Mg-ATPase and Na,K-ATPase in the Test control were significantly ( < .05) lower than those of all the other groups.
Pre-treatment with the extracts and metformin boosted endogenous antioxidants, and prevented doxorubicin-induced renal damage, as indicated by the attenuation of doxorubicin-induced renal oxidative stress, as well as the attenuation of doxorubicin-induced adverse alterations in renal cholesterol, ATPases and electrolyte balance, and plasma biomarkers of kidney function, and keeping them at near-normal values.
阿霉素用于化疗的主要缺点是其对包括肾脏在内的各种器官具有毒性。本研究调查了[植物名称1]和[植物名称2]水提物对阿霉素诱导的肾毒性的潜在保护作用。
为此，在阿霉素处理的大鼠中监测了它们对肾功能血浆生物标志物、肾脂质谱、氧化应激生物标志物、电解质谱和肾ATP酶活性的影响。二甲双胍（250毫克/千克体重，口服）和提取物（50、75和100毫克/千克，口服）每天给药14天；而在研究的第12天，一次性腹腔注射阿霉素（15毫克/千克）诱导肾毒性。
试验对照组中肌酐、尿素的血浆浓度以及肾脏丙二醛、胆固醇、钙和钠的浓度显著（P<0.05）高于所有其他组。然而，试验对照组中肾脏抗坏血酸、氯、镁和钾的浓度以及过氧化氢酶、谷胱甘肽过氧化物酶、超氧化物歧化酶、钙ATP酶、镁ATP酶和钠钾ATP酶的肾脏活性显著（P<0.05）低于所有其他组。
提取物和二甲双胍预处理可增强内源性抗氧化剂，并预防阿霉素诱导的肾损伤，这表现为阿霉素诱导的肾氧化应激减轻，以及阿霉素诱导的肾脏胆固醇、ATP酶和电解质平衡以及肾功能血浆生物标志物的不良改变减轻，并使其保持在接近正常的值。
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