Andriana Yusuf, Xuan Tran Dang, Quy Tran Ngoc, Minh Truong Ngoc, Van Truong Mai, Viet Tran Duc
Graduate School for International Development and Cooperation, Hiroshima University, Hiroshima 739-8529, Japan.
Development Center for Appropriate Technology, Indonesian Institute of Sciences, Jl. KS. Tubun No. 5 Subang, Jawa Barat 41213, Indonesia.
Foods. 2019 Jan 10;8(1):21. doi: 10.3390/foods8010021.
L. is a medicinal plant and used as a drink to treat bronchial catarrh, diarrhea, dysentery and liver diseases. In this study, we evaluated the potential use of to treat hyperuricemia, oxidative stress, and bacterial infection. Ethyl acetate extract of this plant was separated to different fractions by column chromatography (CC) using chloroform and methanol as eluents and subjected to xanthine oxidase (XO) inhibitory, antioxidant, and antibacterial assays. The results showed that the F fraction exhibited the strongest XO inhibitory activity (IC = 133.17 µg/mL), while the F fraction possessed maximum antioxidant activity assessed by DPPH (2,2-diphenyl-2-picrylhydrazyl) and ABTS (2,2'-azinobis (3-ethylbenzothiazoline-6-sulfonic acid) assays (IC = 0.51 and 1.04 mg/mL, respectively). In addition, the F fraction presented the most effective inhibition on the growth of , , , and . Gas chromatography-mass spectrophotometry (GS-MS) and liquid chromatography-electrospray ionization-mass spectrophotometry (LC-ESI-MS) results revealed that fatty acids, glycerides, and flavonoids were the major compounds of the F fraction. Glycerides, triose sugar alcohols, and fatty acids were dominant compounds of the F fraction, while sterols were principal components of the F fraction. This study indicated that had potent inhibitory effects on XO inhibitory, antioxidant, and antibacterial activities. These biological activities may be attributed to the presence of fatty acids, flavonoids, and sterols in this plant. It is suggested that can be utilized as a healthy source to develop beverages and foods to treat antihyperuricemia, oxidative stress, and bacterial infection.
L.是一种药用植物，用作治疗支气管粘膜炎、腹泻、痢疾和肝脏疾病的饮品。在本研究中，我们评估了其治疗高尿酸血症、氧化应激和细菌感染的潜在用途。该植物的乙酸乙酯提取物通过柱色谱法（CC）以氯仿和甲醇作为洗脱剂分离成不同馏分，并进行黄嘌呤氧化酶（XO）抑制、抗氧化和抗菌测定。结果表明，F馏分表现出最强的XO抑制活性（IC = 133.17 µg/mL），而通过DPPH（2,2-二苯基-2-苦基肼）和ABTS（2,2'-偶氮二（3-乙基苯并噻唑啉-6-磺酸）测定评估，F馏分具有最大的抗氧化活性（IC分别为0.51和1.04 mg/mL）。此外，F馏分对[具体细菌名称未给出]、[具体细菌名称未给出]、[具体细菌名称未给出]和[具体细菌名称未给出]的生长呈现出最有效的抑制作用。气相色谱-质谱法（GS-MS）和液相色谱-电喷雾电离-质谱法（LC-ESI-MS）结果显示，脂肪酸、甘油酯和黄酮类化合物是F馏分的主要成分。甘油酯、三糖糖醇和脂肪酸是F馏分的主要化合物，而甾醇是F馏分的主要成分。本研究表明，L.对XO抑制、抗氧化和抗菌活性具有显著的抑制作用。这些生物活性可能归因于该植物中脂肪酸、黄酮类化合物和甾醇的存在。建议L.可作为一种健康来源，用于开发治疗高尿酸血症、氧化应激和细菌感染的饮料和食品。
