College of Horticulture and Plant Protection, Yangzhou University, Yangzhou 225009, China.
College of Horticulture and Plant Protection, Yangzhou University, Yangzhou 225009, China; Joint International Research Laboratory of Agriculture and Agri-Product Safety, the Ministry of Education of China, Yangzhou University, Yangzhou 225009, China.
Food Chem. 2020 Jul 30;319:126548. doi: 10.1016/j.foodchem.2020.126548. Epub 2020 Mar 4.
Paeonia ostii is an emerging woody oil crop and cultivated on a large scale in China. This study attempted to characterize the characteristics of P. ostii seed oil body (OB) and found the key genes related to its morphology. The results showed that P. ostii seed oil contained five dominant fatty acid compositions that were stored in OBs. During the development of seeds, oil yield and fatty acid content were in line with OB accumulation. Moreover, NaCl concentration and pH value could influence P. ostii OB stability. Additionally, oleosins (OLEs) were identified as the most abundant proteins in OBs. Based on the expression levels of OLEs, PoOLE17.5 was isolated, which might be localized in OBs. And overexpression of PoOLE17.5 in tobacco obviously increased seed size and hundred-seed weight, altered OB morphology and increased fatty acid content. These results could provide a theoretical basis for improving oil yield of P. ostii seeds.
牡丹籽油是一种新兴的木本油料作物，在中国已大规模种植。本研究旨在对牡丹籽油体（OB）的特性进行鉴定，并找到与它的形态相关的关键基因。结果表明，牡丹籽油中含有五种主要的脂肪酸组成，它们被储存在 OB 中。在种子发育过程中，产油量和脂肪酸含量与 OB 的积累相一致。此外，NaCl 浓度和 pH 值会影响牡丹籽油体的稳定性。此外，油体蛋白（OLEs）被鉴定为 OB 中最丰富的蛋白质。根据 OLEs 的表达水平，分离出 PoOLE17.5，它可能定位于 OB 中。在烟草中过表达 PoOLE17.5 明显增大了种子的大小和百粒重，改变了 OB 的形态并增加了脂肪酸的含量。这些结果可为提高牡丹籽油产量提供理论依据。
