Brubaker D B, Romine C M
Harbor-UCLA Medical Center, Torrance.
Transfusion. 1988 Jul-Aug;28(4):383-5. doi: 10.1046/j.1537-2995.1988.28488265273.x.
White cell-poor blood components are useful in patients with white cell antibodies. White cells are efficiently removed by two different filters, Imugard and Erypur, which have used saline as the filter solution. This study evaluated these filters as to their production of white cell-poor platelets. Pools of random-donor platelet concentrates were filtered. Prefiltration and postfiltration samples were evaluated for percentages of platelet recovery, white cell (WBC) removal, and platelet function. The two filter solutions tested were normal-strength saline (NSS) and fresh-frozen plasma (FFP). Postfiltration samples using NSS showed no measurable platelet aggregation with ADP, epinephrine, or collagen. However, with FFP, both filters showed 100 percent platelet aggregation with ADP, epinephrine, and collagen. The FFP filter solution provided excellent white cell removal in both filters (Imugard: 100% WBC removal or less than 1.0 X 10(6) residual WBC; Erypur: 99.5% removal or greater than 1.0 X 10(7) residual WBC); however, platelet recovery was better with Imugard (95%) than with Erypur (55%). The filtration procedure is an excellent method for the preparation of white cell-poor platelets; however, the quantity of the saline solution recommended for the filtering of red cells must be minimized for platelets.
少白细胞血液成分对有白细胞抗体的患者有用。白细胞可通过两种不同的过滤器（Imugard和Erypur）有效去除，这两种过滤器都使用生理盐水作为过滤溶液。本研究评估了这些过滤器制备少白细胞血小板的情况。对随机供者血小板浓缩物池进行过滤。对过滤前和过滤后的样本进行血小板回收率、白细胞（WBC）去除率和血小板功能百分比的评估。测试的两种过滤溶液分别是等渗生理盐水（NSS）和新鲜冰冻血浆（FFP）。使用NSS的过滤后样本与ADP、肾上腺素或胶原均未显示出可测量的血小板聚集。然而，使用FFP时，两种过滤器与ADP、肾上腺素和胶原均显示100%的血小板聚集。FFP过滤溶液在两种过滤器中均能实现出色的白细胞去除（Imugard：100%白细胞去除或残留白细胞少于1.0×10⁶；Erypur：99.5%去除或残留白细胞大于1.0×10⁷）；然而，Imugard的血小板回收率（95%）高于Erypur（55%）。过滤程序是制备少白细胞血小板的一种出色方法；然而，对于血小板而言，推荐用于红细胞过滤的生理盐水溶液量必须减至最少。
