Davies C, Symons R H
Department of Biochemistry, University of Adelaide, Australia.
Virology. 1988 Jul;165(1):216-24. doi: 10.1016/0042-6822(88)90675-7.
The nucleotide sequence of the RNA 3 of the Q-strain of cucumber mosaic virus (Q-CMV) has been reinvestigated and supporting partial amino acid sequence data obtained for the coat protein. Corrections to the previously published sequence of RNA 3 [A. R. Gould and R. H. Symons (1982) Eur. J. Biochem. 126, 217-226] result in changes to the size and composition of the putative 3a and coat proteins. Analysis of the nucleotide sequence revealed a 14-nucleotide sequence present in the intercistronic regions of the RNA 3 molecules of both Q-CMV and brome mosaic virus (BMV). This sequence, which is closely related to sequences previously detected in the 5'-untranslated region of Q-CMV and BMV RNAs 1 and 2 [M. A. Rezaian, R. H. V. Williams, and R. H. Symons (1985) Eur. J. Biochem. 150, 331-339], may be important in the control of RNA synthesis. Computer-assisted comparisons indicate an ancestral relationship between the 3a proteins of CMV, BMV, and alfalfa mosaic virus (AMV) and between the coat proteins of CMV and BMV. These comparisons significantly extend previous observations regarding the close evolutionary relationships within the plant tripartite virus group.
对黄瓜花叶病毒Q株系（Q-CMV）的RNA 3的核苷酸序列进行了重新研究，并获得了衣壳蛋白的部分氨基酸序列数据作为支持。对先前发表的RNA 3序列[ A. R. 古尔德和R. H. 西蒙斯（1982年），《欧洲生物化学杂志》126卷，217 - 226页]的修正导致了假定的3a蛋白和衣壳蛋白的大小及组成发生变化。对核苷酸序列的分析揭示，在Q-CMV和雀麦花叶病毒（BMV）的RNA 3分子的顺反子间区域存在一个14个核苷酸的序列。该序列与先前在Q-CMV以及BMV的RNA 1和RNA 2的5'-非翻译区检测到的序列密切相关[ M. A. 雷扎安、R. H. V. 威廉姆斯和R. H. 西蒙斯（1985年），《欧洲生物化学杂志》150卷，331 - 339页]，可能在RNA合成的控制中起重要作用。计算机辅助比较表明，CMV、BMV和苜蓿花叶病毒（AMV）的3a蛋白之间以及CMV和BMV的衣壳蛋白之间存在祖先关系。这些比较显著扩展了先前关于植物三分体病毒组内密切进化关系的观察结果。
