Rezaian M A, Williams R H, Gordon K H, Gould A R, Symons R H
Eur J Biochem. 1984 Sep 3;143(2):277-84. doi: 10.1111/j.1432-1033.1984.tb08370.x.
The nucleotide sequence of the 3035 residues of RNA 2 (Mr 1.03 X 10(6) ) of the Q strain of cucumber mosaic virus (CMV) was determined by sequencing M13 clones of the RNA 2 cDNA and by dideoxy sequencing using primers prepared either from M13 clones or by chemical synthesis. A single long open reading frame starts at the second AUG from the 5' end of RNA 2 and encodes 839 amino acids (Mr 94333). This frame has flanking regions of 92 nucleotides at the 5' terminus and 423 nucleotides at the 3' terminus. Computer analysis of the nucleotide sequence showed that CMV RNA 2 has a significant homology with RNA 2 of brome mosaic virus (BMV) and alfalfa mosaic virus (AMV) and also with a region for tobacco mosaic virus (TMV) RNA encoding the read-through part of the 183-kDa protein. About 400 amino acids in the central region of the CMV RNA 2 translation product have a striking homology with the corresponding proteins encoded by BMV and AMV and with the read-through part of the TMV 183-kDa protein. Hydrophobicity plots of CMV and BMV RNA translation products also had apparent similarities. It is concluded that CMV is related to BMV, AMV and TMV in order of increasing evolutionary divergence.
通过对黄瓜花叶病毒（CMV）Q株系RNA 2的cDNA的M13克隆进行测序，以及使用从M13克隆制备或化学合成的引物进行双脱氧测序，确定了RNA 2（Mr 1.03 X 10(6)）3035个残基的核苷酸序列。一个单一的长开放阅读框从RNA 2 5'端的第二个AUG开始，编码839个氨基酸（Mr 94333）。该阅读框在5'末端有92个核苷酸的侧翼区域，在3'末端有423个核苷酸的侧翼区域。对核苷酸序列的计算机分析表明，CMV RNA 2与雀麦花叶病毒（BMV）和苜蓿花叶病毒（AMV）的RNA 2以及烟草花叶病毒（TMV）RNA编码183-kDa蛋白通读部分的区域具有显著的同源性。CMV RNA 2翻译产物中心区域约400个氨基酸与BMV和AMV编码的相应蛋白质以及TMV 183-kDa蛋白的通读部分具有显著的同源性。CMV和BMV RNA翻译产物的疏水性图谱也有明显的相似之处。得出的结论是，按照进化差异增加的顺序，CMV与BMV、AMV和TMV相关。
