Hayakawa T, Mizukami M, Nakajima M, Suzuki M
Plantech Research Institute, c/o MKC Research Center, Yokohama, Japan.
J Gen Virol. 1989 Feb;70 ( Pt 2):499-504. doi: 10.1099/0022-1317-70-2-499.
The complete nucleotide sequence (2217 residues) of RNA 3 of cucumber mosaic virus strain O (CMV-O) was determined. Two open reading frames were identified, encoding a 3A protein (279 amino acid residues) in the 5'-proximal region and a coat protein (218 amino acid residues). The amino acid sequence of the coat protein C terminus was determined directly from purified protein, and confirmed the presence of the coat protein open reading frame in CMV-O RNA 3. Comparison of nucleotide sequences and amino acid sequences of CMV strains O, Q, D and Y indicated the close relationship between these strains. A tRNA-like structure could be adopted by the 3' non-coding region, and this resembled a similar structure in CMV-Q in spite of nucleotide substitutions or deletions.
测定了黄瓜花叶病毒O株系（CMV-O）RNA 3的完整核苷酸序列（2217个残基）。鉴定出两个开放阅读框，在5'近端区域编码一个3A蛋白（279个氨基酸残基）和一个外壳蛋白（218个氨基酸残基）。外壳蛋白C末端的氨基酸序列是直接从纯化蛋白中确定的，并证实了CMV-O RNA 3中存在外壳蛋白开放阅读框。CMV O、Q、D和Y株系的核苷酸序列和氨基酸序列比较表明这些株系之间关系密切。3'非编码区可形成类似tRNA的结构，尽管存在核苷酸取代或缺失，但这与CMV-Q中的类似结构相似。
