一阶导数分光光度法和气液色谱法测定食品和药品中的咖啡因。I. 咖啡、茶和软饮料中咖啡因的快速测定。

First-derivative spectrophotometric and gas-liquid chromatographic determination of caffeine in foods and pharmaceuticals. I. Rapid determination of caffeine in coffee, tea and soft drinks.

作者信息

Abdel-Moety E M

机构信息

Department of Analytical Chemistry, Faculty of Pharmacy, Cairo University, Kasr El-Aini, Egypt.

出版信息

Z Lebensm Unters Forsch. 1988 May;186(5):412-6. doi: 10.1007/BF01127301.

DOI:10.1007/BF01127301

PMID:3388978

Abstract

A simple and rapid spectrophotometric method is described for the determination of caffeine in coffee, tea and soft drinks. To exclude the irrelevant absorptions, due to the presence of some accompanying naturally occurring ingredients in the analysed multicomponent drinks, the derivative method is found to be superior. Another gas-liquid chromatographic (GLC) procedure for the quantitation of the analeptic agent, in bulk form and in the same drinks, is also investigated. The recovery testing of the proposed methods gives good percentage mean recoveries with relatively low deviations. The results of the methods described are compared with those obtained by direct UV-spectrophotometry as well as by applying an official pharmacopoeial procedure for caffeine.

摘要

本文描述了一种简单快速的分光光度法，用于测定咖啡、茶和软饮料中的咖啡因。为排除由于被分析的多组分饮料中存在一些天然伴生成分而产生的无关吸收，发现导数法更为优越。还研究了另一种气相色谱（GLC）方法，用于定量散装形式以及相同饮料中的兴奋药。所提出方法的回收率测试给出了良好的平均回收率百分比，偏差相对较低。将所述方法的结果与通过直接紫外分光光度法以及应用咖啡因的官方药典程序获得的结果进行了比较。