高效液相色谱法测定脱咖啡因咖啡、茶及饮料产品中的咖啡因。
High performance liquid chromatographic determination of caffeine in decaffeinated coffee, tea, and beverage products.
作者信息
Ashoor S H, Seperich G J, Monte W C, Welty J
出版信息
J Assoc Off Anal Chem. 1983 May;66(3):606-9.
PMID:6863182
Abstract
A method was developed for determining caffeine in decaffeinated coffee, tea, and beverage products by high performance liquid chromatography (HPLC). The HPLC system consisted of a Bio-Sil ODS-5S C18 column, methanol-water (25 + 75) mobile phase at 1 mL/min, and a UV detector. The method is simple and specific. Caffeine recoveries were 93.8-98.3% and coefficients of variation were 0.90-2.25%.
摘要
开发了一种通过高效液相色谱法（HPLC）测定脱咖啡因咖啡、茶和饮料产品中咖啡因的方法。HPLC系统由Bio-Sil ODS-5S C18柱、流速为1 mL/min的甲醇-水（25 + 75）流动相和紫外检测器组成。该方法简单且具有特异性。咖啡因回收率为93.8 - 98.3%，变异系数为0.90 - 2.25%。
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