免疫工程化的磁性量子点纳米珠系统，用于分离和检测循环肿瘤细胞。

Immunoengineered magnetic-quantum dot nanobead system for the isolation and detection of circulating tumor cells.

机构信息

Department of Pharmacy, Changzheng Hospital, Second Military Medical University, Shanghai, 200003, China.

Department of Central Laboratory, Shanghai Skin Disease Hospital, Tongji University School of Medicine, Shanghai, 200443, China.

出版信息

J Nanobiotechnology. 2021 Apr 23;19(1):116. doi: 10.1186/s12951-021-00860-1.

BACKGROUND

Highly efficient capture and detection of circulating tumor cells (CTCs) remain elusive mainly because of their extremely low concentration in patients' peripheral blood.

METHODS

We present an approach for the simultaneous capturing, isolation, and detection of CTCs using an immuno-fluorescent magnetic nanobead system (iFMNS) coated with a monoclonal anti-EpCAM antibody.

RESULTS

The developed antibody nanobead system allows magnetic isolation and fluorescent-based quantification of CTCs. The expression of EpCAM on the surface of captured CTCs could be directly visualized without additional immune-fluorescent labeling. Our approach is shown to result in a 70-95% capture efficiency of CTCs, and 95% of the captured cells remain viable. Using our approach, the isolated cells could be directly used for culture, reverse transcription-polymerase chain reaction (RT-PCR), and immunocytochemistry (ICC) identification. We applied iFMNS for testing CTCs in peripheral blood samples from a lung cancer patient.

CONCLUSIONS

It is suggested that our iFMNS approach would be a promising tool for CTCs enrichment and detection in one step.

背景

高效捕获和检测循环肿瘤细胞（CTCs）仍然难以实现，主要是因为它们在患者外周血中的浓度极低。

方法

我们提出了一种使用单克隆抗 EpCAM 抗体涂覆的免疫荧光磁性纳米珠系统（iFMNS）同时捕获、分离和检测 CTCs 的方法。

结果

开发的抗体纳米珠系统允许通过磁性分离和荧光定量来检测 CTCs。捕获的 CTCs 表面上的 EpCAM 表达无需额外的免疫荧光标记即可直接可视化。我们的方法显示 CTCs 的捕获效率为 70-95%，95%的捕获细胞保持存活。使用我们的方法，可以直接将分离的细胞用于培养、逆转录-聚合酶链反应（RT-PCR）和免疫细胞化学（ICC）鉴定。我们应用 iFMNS 测试了肺癌患者外周血样本中的 CTCs。