Clinic of Oral Medicine and Orofacial Pain, Institute of Oral Health Science, Ajou University School of Medicine, 164, Worldcup-ro, Yeongtong-gu, Suwon, Gyeonggi-do, 16499, Republic of Korea.
Sci Rep. 2021 Apr 23;11(1):8872. doi: 10.1038/s41598-021-88275-8.
This study aimed to investigate immune-related pathophysiology of the temporomandibular joint (TMJ) osteoarthritis (OA) in young females by analyzing transcriptional profiles of peripheral blood mononuclear cells. The RNA-sequencing (RNA-seq) was conducted on 24 young females with TMJ OA (mean age 19.3 ± 3.1 years) (RNAOA) and 11 age and sex matched healthy controls (mean age 20.5 ± 3.7 years) (CON). RNA-seq datasets were analyzed to identify genes, pathways, and regulatory networks of those which were involved in the development of TMJ OA. RNA-seq data analysis revealed 41 differentially expressed genes (DEGs) between RNAOA and CON. A total of 16 gene ontology (GO) terms including three molecular and 13 biological terms were annotated via the GO function of molecular function and biological process. Through ingenuity pathway analysis (IPA), 21 annotated categories of diseases and functions were identified. There were six hub genes which showed significant results in both GO enrichment analysis and IPA, namely HLA-C, HLA-F, CXCL8, IL11RA, IL13RA1, and FCGR3B. The young females with TMJ OA showed alterations of the genes related to immune function in the blood and some of changes may reflect inflammation, autoimmunity, and abnormal T cell functions.
本研究旨在通过分析外周血单核细胞的转录谱，研究年轻女性颞下颌关节（TMJ）骨关节炎（OA）的免疫相关病理生理学。对 24 名患有 TMJ OA 的年轻女性（平均年龄 19.3±3.1 岁）（RNAOA）和 11 名年龄和性别匹配的健康对照组（平均年龄 20.5±3.7 岁）（CON）进行了 RNA 测序（RNA-seq）。对 RNA-seq 数据集进行分析，以确定参与 TMJ OA 发生的基因、途径和调控网络。RNA-seq 数据分析显示，RNAOA 和 CON 之间有 41 个差异表达基因（DEGs）。通过 GO 功能的分子功能和生物过程注释了总共 16 个基因本体（GO）术语，包括三个分子和 13 个生物学术语。通过 ingenuity pathway analysis（IPA），确定了 21 个注释的疾病和功能类别。有六个枢纽基因在 GO 富集分析和 IPA 中均显示出显著结果，即 HLA-C、HLA-F、CXCL8、IL11RA、IL13RA1 和 FCGR3B。患有 TMJ OA 的年轻女性血液中与免疫功能相关的基因发生了改变，其中一些变化可能反映了炎症、自身免疫和异常 T 细胞功能。
