I. M. Sechenov Institute of Evolutionary Physiology and Biochemistry, Russian Academy of Sciences, St. Petersburg, Russia.
Institute of Experimental Medicine, St. Petersburg, Russia.
Bull Exp Biol Med. 2021 Apr;170(6):714-718. doi: 10.1007/s10517-021-05139-x. Epub 2021 Apr 24.
We studied the effect of different doses of ammonium chloride (ACl) and ammonium carbonate (ACr) on immunological parameters of the peripheral blood in rats during high-intensity exercise. Changes in the absolute and relative numbers of granulocytes, lymphocytes, natural killers, naive and mature effector cells one day after the end of the forced swimming cycle were found by using a hematological analyzer and a flow cytometer. Immunological indicators were analyzed relative to swimming duration on the last day of ultimate load. The revealed changes indicate the onset of the effector phase of the development of the inflammatory processes in the positive control group (physiological saline) and in rats receiving a higher dose of ACr (20 mg/kg), while administration of ACl prevented the development of inflammatory processes and shifts in the physiological balance of lymphocyte subpopulations. Immunological profiling indicates that ACl in a dose of 20 mg/kg most effectively improved physical performance in our forced swimming model.
我们研究了不同剂量的氯化铵（ACl）和碳酸铵（ACr）对高强度运动后大鼠外周血免疫参数的影响。使用血液分析仪和流式细胞仪，在强制游泳周期结束后一天，发现粒细胞、淋巴细胞、自然杀伤细胞、幼稚和成熟效应细胞的绝对值和相对数量发生了变化。免疫指标相对于最后一天的最大负荷游泳时间进行分析。结果表明，在阳性对照组（生理盐水）和接受较高剂量 ACr（20mg/kg）的大鼠中，炎症过程的效应阶段开始，而 ACl 的给药可防止炎症过程的发展和淋巴细胞亚群的生理平衡发生变化。免疫分析表明，在我们的强制游泳模型中，20mg/kg 的 ACl 剂量最有效地提高了运动表现。
