School of Environment, Henan Key Laboratory for Environmental Pollution Control, Key Laboratory for Yellow River and Huai River Water Environmental Pollution Control, Ministry of Education, Henan Normal University, Xinxiang, Henan 453007, PR China.
School of Environment, Henan Key Laboratory for Environmental Pollution Control, Key Laboratory for Yellow River and Huai River Water Environmental Pollution Control, Ministry of Education, Henan Normal University, Xinxiang, Henan 453007, PR China.
Bioresour Technol. 2021 Aug;333:125165. doi: 10.1016/j.biortech.2021.125165. Epub 2021 Apr 20.
This study investigated the effects of garbage enzyme (GE), pelelith (PL), and biochar (BC) on nitrogen (N) conservation, nitrogenase (Nase) and N-fixing bacteria during the composting of sewage sludge. Results showed that the addition of GE, PL, and BC reduced NH emissions by 40.9%, 29.3%, and 67.4%, and increased the NO-N contents of the end compost by 161.4, 88.2, and 105.8% relative to control, respectively, thus increasing the TN content. Three additives improved Nase, cellulase, and fluorescein diacetate hydrolase (FDA) activities and the abundances of nifH gene, and the largest increase was BC, followed by PL and GE. In addition, the additives also markedly influenced the succession of N-fixing bacteria, and significantly increased the abundance of Proteobacteria during the whole process. The BC and PL additions strengthened the sensitivity of N-fixing bacteria to environmental variables, and FDA, TN, moisture content, and NO-N significantly affected the N-fixing bacteria at genus level.
本研究考察了垃圾酶（GE）、胞外酶（PL）和生物炭（BC）对污水污泥堆肥过程中氮素保存、固氮酶（Nase）和固氮菌的影响。结果表明，与对照相比，添加 GE、PL 和 BC 分别减少了 40.9%、29.3%和 67.4%的 NH 排放，使终堆肥的 NO-N 含量分别增加了 161.4%、88.2%和 105.8%，从而增加了 TN 含量。三种添加剂均提高了 Nase、纤维素酶和荧光素二乙酸酯水解酶（FDA）的活性以及 nifH 基因的丰度，其中 BC 的效果最大，其次是 PL 和 GE。此外，这些添加剂还显著影响了固氮菌的演替，并且在整个过程中显著增加了变形菌门的丰度。BC 和 PL 的添加增强了固氮菌对环境变量的敏感性，FDA、TN、水分含量和 NO-N 对固氮菌属水平有显著影响。
