School of Environment, Henan Key Laboratory for Environmental Pollution Control, Key Laboratory for Yellow River and Huai River Water Environmental Pollution Control, Ministry of Education, Henan Normal University, Xinxiang, Henan 453007, PR China.
School of Environment, Henan Key Laboratory for Environmental Pollution Control, Key Laboratory for Yellow River and Huai River Water Environmental Pollution Control, Ministry of Education, Henan Normal University, Xinxiang, Henan 453007, PR China.
Bioresour Technol. 2021 May;327:124792. doi: 10.1016/j.biortech.2021.124792. Epub 2021 Feb 3.
This study evaluated nitrogen transformation, enzymatic activities and bacterial succession during sewage sludge composting with and without garbage enzymes (GE and CK, respectively). The results showed that GE addition significantly increased fluorescein diacetate hydrolase (FDA), cellulase, and nitrogenase activities during the composting process. GE addition reduced the cumulative NH emissions by 66.5%, increased the peak NH-N content by 26.3% and increased the total nitrogen (TN) content of the end compost by 39.2% compared to CK. Microbiological analysis revealed that GE addition significantly increased the relative abundance of Firmicutes during the thermophilic and cooling phases relative to CK. The selected factors affected the bacterial community composition in the following order: NH-N > TOC > FDA > TN > C/N. Network analysis also showed that the enzymes were secreted mainly by Bacillus and norank_f_Caldilineaceae in GE, while they were secreted primarily by norank_f_Methylococcaceae in CK during the composting process.
本研究评估了添加和不添加垃圾酶（GE 和 CK）时污水污泥堆肥过程中的氮转化、酶活性和细菌演替。结果表明，GE 添加显著提高了堆肥过程中的荧光素二乙酸酯水解酶（FDA）、纤维素酶和固氮酶活性。与 CK 相比，GE 添加减少了 66.5%的累积 NH 排放，增加了 26.3%的峰值 NH-N 含量，并增加了最终堆肥的总氮（TN）含量。微生物分析表明，GE 添加在高温和冷却阶段相对于 CK 显著增加了厚壁菌门的相对丰度。所选因素对细菌群落组成的影响顺序为：NH-N>TOC>FDA>TN>C/N。网络分析还表明，在 GE 中，酶主要由芽孢杆菌和 norank_f_Caldilineaceae 分泌，而在 CK 中，酶主要由 norank_f_Methylococcaceae 分泌。
