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生物炭和垃圾酶联合添加改善污水污泥堆肥过程中腐殖化和真菌群落演替。

Combined addition of biochar and garbage enzyme improving the humification and succession of fungal community during sewage sludge composting.

机构信息

School of Environment, Henan Key Laboratory for Environmental Pollution Control, Key Laboratory for Yellow River and Huai River Water Environmental Pollution Control, Ministry of Education, Henan Normal University, Xinxiang, Henan 453007, PR China.

School of Environment, Henan Key Laboratory for Environmental Pollution Control, Key Laboratory for Yellow River and Huai River Water Environmental Pollution Control, Ministry of Education, Henan Normal University, Xinxiang, Henan 453007, PR China.

出版信息

Bioresour Technol. 2022 Feb;346:126344. doi: 10.1016/j.biortech.2021.126344. Epub 2021 Nov 13.

DOI:10.1016/j.biortech.2021.126344
PMID:34780901
Abstract

The influences of combination of garbage enzyme and biochar on total organic carbon (TOC) degradation, humification and the fungal succession during sewage sludge (SS) composting were established. Results showed that the GE and BC + GE treatments significantly increased the enzyme activity of fluorescein diacetate hydrolase (FDA) and increased the TOC degradation rate by 9.8% and 21.9% relative to control. The excitation-emission matrix (EEM) combined with the percentage fluorescence response (Pi, n) also proved that the combination of BC and GE promoted fulvic acid-like and humic-like substances production, and thus increased humification. Furthermore, the combination of BC and GE effectively decreased the relative abundance of Unclassified_k_Fugni, while increased the abundance of Ascomycota and Basidiomycota compared with control. The four genera, Pseudeurotium, Talaromyces, Trichoderma, and Penicillium, were the main fungi for the humification. Comparatively, the combined of BC and GE showed the optimal performance for TOC degradation and humification during SS composting.

摘要

研究了垃圾酶(GE)和生物炭(BC)组合对污水污泥(SS)堆肥过程中总有机碳(TOC)降解、腐殖化和真菌演替的影响。结果表明,GE 和 BC+GE 处理显著提高了荧光素二乙酸酯水解酶(FDA)的酶活性,与对照相比,TOC 降解率分别提高了 9.8%和 21.9%。激发-发射矩阵(EEM)结合荧光百分比响应(Pi,n)也证明,BC 和 GE 的组合促进了富里酸类和腐殖酸类物质的产生,从而提高了腐殖化程度。此外,与对照相比,BC 和 GE 的组合有效降低了未分类_k_Fugni 的相对丰度,而增加了子囊菌门和担子菌门的丰度。四个属,拟青霉属、拟青霉属、木霉属和青霉属,是腐殖化的主要真菌。相比之下,BC 和 GE 的组合在 SS 堆肥过程中对 TOC 降解和腐殖化表现出最佳性能。

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