Department of Orthopedics, The General Hospital of Ningxia Medical University, PR China; Department of Orthopedics, The Autonomous Region People's Hospital of Ningxia Medical University (People's Hospital of Ningxia Hui Autonomous Region), PR China.
Department of Orthopedics, The General Hospital of Ningxia Medical University, PR China.
Cytokine. 2021 Jul;143:155546. doi: 10.1016/j.cyto.2021.155546. Epub 2021 Apr 22.
Osteoarthritis (OA) is a joint disease characterized by articular cartilage loss and afflicts many people worldwide. However, diagnostic methods and treatment options remain limited and are often characterized by low sensitivity and low efficacy. The focus of the present study was to identify proteomic biomarkers in synovial fluid to improve diagnosis and therapy of OA patients.
Antibody array technology was utilized for protein expression profiling of synovial fluid from 24 OA patients and 24 healthy persons.
Compared with healthy persons, twenty proteins showed lower expression levels in OA patients, while thirty proteins had higher levels. Among these differential proteins, GITRL, CEACAM-1, FSH, EG-VEGF, FGF-4, PIGF, Cystatin EM and NT-4 were found for the first time to be differentially expressed in OA. Bioinformatics analysis showed that most of these differential proteins were involved leukocytes events, and some differentially expressed proteins including IL-18, CXCL1, CTLA4, MIP-3b, CD40, MMP-1, THBS1, CCL11, PAI-1, BAFF, aggrecan, angiogenin and follistatin were located in central positions of the protein-protein interaction (PPI) network.
We speculate that leukocyte proliferation and migration to the joint may be an important pathogenesis of OA, which needs a further validation. The central proteins of the PPI network may play a more pivotal role in OA. The newly identified differentially expressed proteins may be novel biomarkers for OA diagnosis and targets for OA therapy.
骨关节炎（OA）是一种以关节软骨丧失为特征的关节疾病，困扰着全世界许多人。然而，诊断方法和治疗选择仍然有限，通常表现出低灵敏度和低疗效。本研究的重点是确定滑液中的蛋白质组生物标志物，以改善 OA 患者的诊断和治疗。
采用抗体芯片技术对 24 例 OA 患者和 24 例健康人的滑液进行蛋白质表达谱分析。
与健康人相比，OA 患者的 20 种蛋白质表达水平较低，而 30 种蛋白质表达水平较高。在这些差异蛋白中，GITRL、CEACAM-1、FSH、EG-VEGF、FGF-4、PIGF、Cystatin EM 和 NT-4 首次被发现在 OA 中差异表达。生物信息学分析表明，这些差异蛋白大多数参与白细胞事件，一些差异表达蛋白，包括 IL-18、CXCL1、CTLA4、MIP-3b、CD40、MMP-1、THBS1、CCL11、PAI-1、BAFF、聚集蛋白聚糖、血管生成素和卵泡抑素，位于蛋白质-蛋白质相互作用（PPI）网络的中心位置。
我们推测白细胞增殖和向关节迁移可能是 OA 的一个重要发病机制，这需要进一步验证。PPI 网络的中心蛋白可能在 OA 中发挥更关键的作用。新鉴定的差异表达蛋白可能是 OA 诊断的新生物标志物和 OA 治疗的靶点。
