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Radioiodination of proteins and lipoproteins using N-bromosuccinimide as oxidizing agent.

作者信息

Sinn H J, Schrenk H H, Friedrich E A, Via D P, Dresel H A

机构信息

Department of Nuclear Medicine, Deutsches Krebsforschungszentrum, Heidelberg, Federal Republic of Germany.

出版信息

Anal Biochem. 1988 Apr;170(1):186-92. doi: 10.1016/0003-2697(88)90107-8.

DOI:10.1016/0003-2697(88)90107-8
PMID:3389510
Abstract

In an attempt to improve conditions for radioiodination of sensitive proteins we used N-bromosuccinimide as a mild oxidizing agent. Under gentle conditions we increased the average labeling efficiency of a wide variety of proteins to above 97%. There was no loss of binding activity of low density lipoprotein particles, which are most sensitive to oxidation. Depending on high labeling efficiency, our method reduces preparation time as well as radioactive waste, costs, and irradiation exposure to personnel.

摘要

相似文献

1
Radioiodination of proteins and lipoproteins using N-bromosuccinimide as oxidizing agent.
Anal Biochem. 1988 Apr;170(1):186-92. doi: 10.1016/0003-2697(88)90107-8.
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Use of N-bromosuccinimide for the iodination of proteins for radioimmunoassay.N-溴代琥珀酰亚胺在蛋白质碘化用于放射免疫测定中的应用。
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Reactivity of the tryptophan residues in bovine pancreatic deoxyribonuclease with N-bromosuccinimide.牛胰脱氧核糖核酸酶中色氨酸残基与N-溴代琥珀酰亚胺的反应活性。
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Iodo-Gen-mediated radioiodination of nucleic acids.碘甘醚介导的核酸放射性碘化
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A versatile procedure for the radioiodination of proteins and labeling reagents.一种用于蛋白质和标记试剂放射性碘化的通用方法。
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Studies of the N-bromosuccinimide inactivation of the enzyme rhodanese.关于硫氰酸酶的N-溴代琥珀酰亚胺失活作用的研究。
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Radioiodination by use of the Bolton-Hunter and related reagents.使用博尔顿-亨特试剂及相关试剂进行放射性碘化。
Methods Enzymol. 1980;70(A):221-47. doi: 10.1016/s0076-6879(80)70052-6.

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