Reay P
Ann Clin Biochem. 1982 Mar;19(Pt 2):129-33. doi: 10.1177/000456328201900214.
A new method, employing the mild oxidant N-bromosuccinimide, for the iodination of proteins for radioimmunoassay is described. Human prolactin, follicle-stimulating, luteinising, thyroid-stimulating, and growth hormones were iodinated with 125I by this method to specific activities up to 136 muCi/microgram. Incorporation of iodine ranged from 67% to 83% using 2 microgram hormone, 200 muCi Na125I, 0.5 nmol oxidant, and a reaction time of 15s. In comparative studies in immunological activity after iodination by this method was equal to, or greater than that by the chlorine gas, Iodo-Gen, or chloramine-T methods. The reagent is cheap and stable, and the method permits efficient use of antigen and iodide.
本文描述了一种采用温和氧化剂N-溴代琥珀酰亚胺进行蛋白质碘化用于放射免疫分析的新方法。用该方法将人催乳素、促卵泡激素、促黄体生成素、促甲状腺激素和生长激素与¹²⁵I进行碘化，比活度高达136μCi/μg。使用2μg激素、200μCi Na¹²⁵I、0.5nmol氧化剂和15秒的反应时间，碘的掺入率在67%至83%之间。在比较研究中，该方法碘化后的免疫活性等于或高于氯气法、碘甘醚法或氯胺-T法。该试剂价格便宜且稳定，该方法能有效利用抗原和碘化物。
