School of Electrical and Electronic Engineering, Yonsei University, Seoul, 03722, South Korea.
Department of Microbiology, Yonsei University College of Medicine, Seoul, 03722, South Korea.
Biosens Bioelectron. 2021 Jul 15;184:113219. doi: 10.1016/j.bios.2021.113219. Epub 2021 Apr 22.
In this report, we investigate plasmon-enhanced imaging fluorescence correlation spectroscopy (p-FCS). p-FCS takes advantage of extreme light confinement by localization at nanogap-based plasmonic nanodimer arrays (PNAs) for enhanced signal-to-noise ratio (SNR) and improved precision by registration with surface plasmon microscopy images. Theoretical results corroborate the enhancement by PNAs in the far-field. Near-field scanning optical microscopy was used to confirm near-field localization experimentally. Experimental confirmation was also conducted with fluorescent nanobeads. The concept was further applied to studying the diffusion dynamics of lysosomes in HEK293T cells stimulated by phorbol 12-myristate 13-acetate treatment. It was found that lysosomes demonstrate stronger super-diffusive behavior with relatively weaker sub-diffusion after stimulation. SNR measured of p-FCS was improved by 9.77 times over conventional FCS. This report is expected to serve as the foundation for an enhanced analytical tool to explore subcellular dynamics.
在本报告中，我们研究了等离子体增强成像荧光相关光谱学（p-FCS）。p-FCS 利用纳米间隙等离子体纳米二聚体阵列（PNAs）的局域极端光限制，通过与表面等离子体显微镜图像配准来提高信噪比（SNR）和提高精度。理论结果证实了远场中 PNAs 的增强。近场扫描光学显微镜用于实验证实近场局域化。还使用荧光纳米珠进行了实验验证。该概念进一步应用于研究 Phorbol 12-myristate 13-acetate 处理刺激后的 HEK293T 细胞溶酶体的扩散动力学。结果发现，溶酶体在刺激后表现出更强的超扩散行为，相对较弱的亚扩散。与传统的 FCS 相比，p-FCS 测量的 SNR 提高了 9.77 倍。本报告有望成为探索亚细胞动力学的增强分析工具的基础。
