Dingeon B, Quenard M T, Tome H
Service de Biochimie, Centre hospitalier, Chambéry.
Ann Biol Clin (Paris). 1988;46(4):263-6.
Using basal isocratic equipment of HPLC and only one step extraction the determination of urinary free cortisol is available in non specialized laboratory. Results are displayed about reliability and specificity of the method. An extraction coefficient of 97.8 per cent, a good practicability in connection with the use of C18 column chromatography and a reagent flow of 0.5 ml/min. enable a low cost of this determination. Reference values are established for a working adult population.
使用高效液相色谱的基础等度设备，仅通过一步萃取，非专业实验室即可测定尿游离皮质醇。文中展示了该方法的可靠性和特异性结果。萃取系数为97.8%，结合使用C18柱色谱具有良好的实用性，试剂流速为0.5毫升/分钟，使得该测定成本较低。为在职成年人群体建立了参考值。
