Medical Science Research Center, State Key Laboratory of Complex Severe and Rare Diseases, Peking Union Medical College Hospital, Chinese Academy of Medical Science and Peking Union Medical College, Beijing, China.
Beijing Key Laboratory for Mechanisms Research and Precision Diagnosis of Invasive Fungal Diseases, Beijing, China.
Anal Sci. 2021 Sep 10;37(9):1275-1279. doi: 10.2116/analsci.20P463. Epub 2021 Apr 23.
Hydrogen peroxide (HO) is a main reactive oxygen by-product produced in the metabolism of organisms and a common biomarker of oxidative stress. Aggregation-induced emission (AIE) probes for HO have been proposed. Such AIEgens mostly use benzeneboronic acid as a recognition group. Recently, a strategy involving enzyme-catalyzed polymerization of AIE compounds shows great potential in AIEgens design. We herein modify the AIE motif, tetraphenylethene (TPE) with o-phenylenediamine (TPE-TAF), which can be oxidated by HO in HRP to form an intramolecular phenazine structure. Compared with a similar approach, the proposed strategy is simple and the TPE-TAF showed a sensitive "turn-on" fluorescence with HO. The detection limit (LOD) is 3.39 μM and the probe is highly specific against HO. We further verified the reaction mechanism of the enzyme-catalyzed coupling reaction. The probe is a promising candidate as a stable and safe fluorescent substrate in HO sensing.
过氧化氢 (HO) 是生物体代谢过程中产生的主要活性氧副产物，也是氧化应激的常见生物标志物。已经提出了用于 HO 的聚集诱导发射 (AIE) 探针。此类 AIE 试剂大多使用苯硼酸作为识别基团。最近，涉及 AIE 化合物的酶催化聚合的策略在 AIE 试剂设计中显示出巨大的潜力。我们在此用邻苯二胺（TPE-TAF）修饰 AIE 基序四苯乙烯（TPE），HO 在 HRP 中可将其氧化形成分子内吩嗪结构。与类似的方法相比，所提出的策略简单，TPE-TAF 对 HO 表现出灵敏的“开启”荧光。检测限 (LOD) 为 3.39 μM，探针对 HO 具有高度特异性。我们进一步验证了酶催化偶联反应的反应机制。该探针作为 HO 传感的稳定且安全的荧光底物具有广阔的应用前景。
