Key Laboratory for Advanced Materials, School of Chemistry & Molecular Engineering , East China University of Science & Technology , No. 130 Meilong Road , Shanghai 200237 , China.
ACS Appl Mater Interfaces. 2018 Apr 18;10(15):12217-12261. doi: 10.1021/acsami.7b14343. Epub 2017 Nov 28.
Luminescent bioprobes are powerful analytical means for biosensing and optical imaging. Luminogens featured with aggregation-induced emission (AIE) attributes have emerged as ideal building blocks for high-performance bioprobes. Bioprobes constructed with AIE luminogens have been identified to be a novel class of FL light-up probing tools. In contrast to conventional bioprobes based on the luminophores with aggregation-caused quenching (ACQ) effect, the AIE-based bioprobes enjoy diverse superiorities, such as lower background, higher signal-to-noise ratio and sensitivity, better accuracy, and more outstanding resistance to photobleaching. AIE-based bioprobes have been tailored for a vast variety of purposes ranging from biospecies sensing to bioimaging to theranostics (i.e., image-guided therapies). In this review, recent five years' advances in AIE-based bioprobes are briefly overviewed in a perspective distinct from other reviews, focusing on the most appealing trends and progresses in this flourishing research field. There are altogether 11 trends outlined, which have been classified into four aspects: the probe composition and form (bioconjugtes, nanoprobes), the output signal of probe (far-red/near-infrared luminescence, two/three-photon excited fluorescence, phosphorescence), the modality and functionality of probing system (dual-modality, dual/multifunctionality), the probing object and application outlet (specific organelles, cancer cells, bacteria, real samples). Typical examples of each trend are presented and specifically demonstrated. Some important prospects and challenges are pointed out as well in the hope of intriguing more interests from researchers working in diverse areas into this exciting research field.
发荧光生物探针是用于生物传感和光学成像的有力分析手段。具有聚集诱导发光（AIE）特性的发光团已成为高性能生物探针的理想构建模块。用 AIE 发光团构建的生物探针被确定为一类新型的 FL 点亮探测工具。与基于具有聚集猝灭（ACQ）效应的荧光团的传统生物探针相比，基于 AIE 的生物探针具有多种优势，例如更低的背景、更高的信噪比和灵敏度、更好的准确性以及更出色的抗光漂白性。基于 AIE 的生物探针已针对从生物物种传感到生物成像到治疗（即图像引导治疗）等各种用途进行了定制。在本综述中，从不同于其他综述的角度简要概述了最近五年基于 AIE 的生物探针的进展，重点介绍了这一蓬勃发展的研究领域中最吸引人的趋势和进展。共概述了 11 种趋势，分为四个方面：探针组成和形式（生物缀合物、纳米探针）、探针的输出信号（远红/近红外发光、双/三光子激发荧光、磷光）、探测系统的模态和功能（双模态、双/多功能）、探测对象和应用出口（特定细胞器、癌细胞、细菌、实际样本）。展示了每种趋势的典型示例，并进行了具体说明。还指出了一些重要的前景和挑战，希望能引起不同领域研究人员对这一令人兴奋的研究领域的更多兴趣。
