Jin Kaidi, Jin Qianqian, Cai Zhongqiang, Huang Baoyu, Wei Lei, Zhang Meiwei, Guo Wen, Liu Yaqiong, Wang Xiaotong
School of Agriculture, Ludong University, Yantai, China.
Changdao Enhancement and Experiment Station, Chinese Academy of Fishery Sciences, Changdao, China.
Front Physiol. 2021 Apr 8;12:666842. doi: 10.3389/fphys.2021.666842. eCollection 2021.
Retinoic acid (RA) signaling pathways mediated by RA receptors (RARs) are essential for many physiological processes such as organ development, regeneration, and differentiation in animals. Recent studies reveal that RARs identified in several mollusks, including Pacific oyster , have a different function mechanism compared with that in chordates. In this report, we identified the molecular characteristics of CgRAR to further explore the mechanism of RAR in mollusks. RT-qPCR analysis shows that CgRAR has a higher expression level in the hemocytes and gonads, indicating that CgRAR may play roles in the processes of development and metabolism. The mRNA expression level of both CgRAR and CgRXR was analyzed by RT-qPCR after injection with RA. The elevated expression of and was detected upon all--RA (ATRA) exposure. Finally, according to the results of Yeast Two-Hybrid assay and co-immunoprecipitation analysis, CgRAR and CgRXR can interact with each other through the C-terminal region. Taken together, our results suggest that CgRAR shows a higher expression level in gonads and hemocytes. ATRA exposure up-regulates the expression of CgRAR and CgRXR. Besides, CgRAR can interact with CgRXR to form a heterodimer complex.
由视黄酸受体（RARs）介导的视黄酸（RA）信号通路对于动物的许多生理过程至关重要，如器官发育、再生和分化。最近的研究表明，在包括太平洋牡蛎在内的几种软体动物中鉴定出的RARs与脊索动物中的RARs具有不同的功能机制。在本报告中，我们鉴定了CgRAR的分子特征，以进一步探索软体动物中RAR的机制。RT-qPCR分析表明，CgRAR在血细胞和性腺中具有较高的表达水平，表明CgRAR可能在发育和代谢过程中发挥作用。注射RA后，通过RT-qPCR分析CgRAR和CgRXR的mRNA表达水平。在全反式视黄酸（ATRA）暴露后，检测到两者的表达升高。最后，根据酵母双杂交试验和免疫共沉淀分析的结果，CgRAR和CgRXR可以通过C末端区域相互作用。综上所述，我们的结果表明CgRAR在性腺和血细胞中表现出较高的表达水平。ATRA暴露上调CgRAR和CgRXR的表达。此外，CgRAR可以与CgRXR相互作用形成异二聚体复合物。
