Qin Wei, Xie Lihui, Li Yongpeng, Liu Hang, Fu Xueqing, Chen Tiantian, Hassani Danial, Li Ling, Sun Xiaofen, Tang Kexuan
Joint International Research Laboratory of Metabolic and Developmental Sciences, Key Laboratory of Urban Agriculture (South) Ministry of Agriculture, Plant Biotechnology Research Center, Fudan-SJTU-Nottingham Plant Biotechnology R&D Center, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, China.
Front Plant Sci. 2021 Apr 9;12:657156. doi: 10.3389/fpls.2021.657156. eCollection 2021.
L. is known for its specific product "artemisinin" which is an active ingredient for curing malaria. Artemisinin is secreted and accumulated in the glandular secretory trichomes (GSTs) on leaves. Earlier studies have shown that increasing GST density is effective in increasing artemisinin content. However, the mechanism of GST initiation is not fully understood. To this end, we isolated and characterized an R2R3-MYB gene, , which is expressed specifically in the GSTs of shoot tips. Overexpression of in increased GST density and enhanced the artemisinin content, whereas RNA interference of resulted in the reduction of GST density and artemisinin content. Additionally, neither overexpression lines nor RNAi lines showed an abnormal phenotype in plant growth and the morphology of GSTs. Our study demonstrates that AaMYB17 is a positive regulator of GSTs' initiation, without influencing the trichome morphology.
黄花蒿以其特定产物“青蒿素”而闻名，青蒿素是治疗疟疾的一种活性成分。青蒿素在叶片的腺毛状分泌毛（GSTs）中分泌和积累。早期研究表明，增加GST密度可有效提高青蒿素含量。然而，GST起始的机制尚未完全了解。为此，我们分离并鉴定了一个R2R3-MYB基因，该基因在茎尖的GSTs中特异性表达。在烟草中过表达该基因可增加GST密度并提高青蒿素含量，而对该基因进行RNA干扰则导致GST密度和青蒿素含量降低。此外，过表达系和RNAi系在植物生长和GST形态方面均未表现出异常表型。我们的研究表明，AaMYB17是GST起始的正调控因子，且不影响腺毛形态。
