来自楠榜省的绿 () 对感染细菌的鸡体内自由基的影响。

Green () from Lampung province effect toward free radicals in chickens infected with bacteria.

机构信息

Laboratory of Microbiology and Immunology, Faculty of Veterinary Medicine, Brawijaya University, Malang, Indonesia.

Animal Product Technology Laboratory, Faculty of Animal Science, Brawijaya University, Malang, Indonesia.

出版信息

Open Vet J. 2021 Jan-Mar;11(1):61-69. doi: 10.4314/ovj.v11i1.10. Epub 2021 Jan 27.

DOI:10.4314/ovj.v11i1.10

PMID:33898285

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8057217/

Abstract

BACKGROUND

Foodborne diseases are caused by acquired pathogenic bacteria such as . It causes an intestinal imbalance and the microbial toxins found in the gastrointestinal tract induce symptoms such as diarrhea. Coffee contains active ingredients such as antioxidants and is used as an anti-inflammatory agent by reducing pro-inflammatory cytokine levels in the body.

AIM

The purpose of this study was to determine the interaction between Lampung's robusta coffee and tissue damage in chickens infected by .

METHODS

This study used first-day-old Isa brown layer chickens ( = 60), which were divided into five treatment groups. The negative control group consisted of healthy and normal chickens, whereas the positive control group consisted of chickens infected with bacteria at a concentration of 10 CFU/ml. Groups T1, T2, and T3 were given coffee extract with doses of 500 mg/kg BW (low dose), 1,000 mg/kg BW (moderate dose), and 1,500 mg/kg BW (high dose), respectively, and then infected with bacteria at a concentration of 10 CFU/ml. The coffee extract and bacteria were given orally via a feeding tube at a volume of 0.5 ml per chick. The extract was given for 14 days (from day 3 to day 16), and the bacteria were given on days 16 and 17. On day 18, the chickens were necropsied. The malondialdehyde (MDA) level was analyzed using one-way analysis of variance test with the GLM procedure (<0.05), while the tissue histopath was analyzed using a descriptive qualitative study to examine the ileal damage.

RESULTS

The results showed that the MDA levels (nmol/l) decreased in treatment groups T1, T2, and T3 compared to the positive control. On the contrary, we found improvements in the ileum histopathology of group T1 and T2 in the form of normal and regular intestinal epithelium arrangement of the ileum, long intestinal villi, and decreased total leukocytes.

CONCLUSION

Green coffee robusta has the potential to increase antioxidants and reduce inflammation in the small intestine of chickens infected with .

摘要

背景

食源性疾病是由获得性病原菌如引起的。它会导致肠道失衡，胃肠道中发现的微生物毒素会引起腹泻等症状。咖啡含有抗氧化剂等活性成分，通过降低体内促炎细胞因子水平，可用作抗炎剂。

目的

本研究旨在确定兰邦罗布斯塔咖啡与感染鸡组织损伤之间的相互作用。

方法

本研究使用了 1 日龄的 Isa brown 层鸡（=60），将其分为五组处理。阴性对照组由健康正常的鸡组成，而阳性对照组由 10 CFU/ml 浓度的细菌感染的鸡组成。T1、T2 和 T3 组分别给予 500mg/kg BW（低剂量）、1000mg/kg BW（中剂量）和 1500mg/kg BW（高剂量）的咖啡提取物，然后用 10 CFU/ml 浓度的细菌感染。通过喂食管给予咖啡提取物和细菌，每只鸡 0.5ml 体积。提取物在 14 天内给予（从第 3 天到第 16 天），细菌在第 16 天和第 17 天给予。第 18 天，对鸡进行尸检。采用单因素方差分析检验（<0.05）分析丙二醛（MDA）水平，同时采用描述性定性研究分析回肠组织病理，检查回肠损伤。