Polk H C, George C D, Hershman M J, Wellhausen S R, Cheadle W G
Price Institute for Surgical Research, Department of Surgery, University of Louisville School of Medicine, Kentucky 40292.
Ann Surg. 1988 Jun;207(6):686-92. doi: 10.1097/00000658-198806000-00007.
The opsonic capacity of patient serum was studied in 43 trauma patients of whom 13 recovered uneventfully, 21 developed major infection, and nine died, mostly of infection. Blood samples were taken within 24 hours of injury. Fifteen patients were studied serially of whom 14 developed severe infection and/or died. Opsonic capacity was determined by flow cytometry and measured as the ability of normal neutrophils to phagocytose killed bacteria previously incubated with patient serum. The most dilute sera reflected changes for better and worse most clearly. On initial assessment, those who died of sepsis showed a 61% mean fluorescent intensity (MFI), which was significantly lower than the 99% MFI for those who survived infection (p less than 0.01) and the 78% MFI of those who developed no infection (p less than 0.05). Serial samples demonstrated a super serum response in four of seven patients surviving major sepsis but in none of the seven who died of infection.
对43例创伤患者的患者血清调理吞噬能力进行了研究，其中13例顺利康复，21例发生严重感染，9例死亡，多数死于感染。在受伤后24小时内采集血样。对15例患者进行了连续研究，其中14例发生严重感染和/或死亡。通过流式细胞术测定调理吞噬能力，并将其作为正常中性粒细胞吞噬预先与患者血清孵育的死细菌的能力进行测量。最稀释的血清最清楚地反映了病情的好转和恶化。在初始评估时，死于败血症的患者平均荧光强度（MFI）为61%，显著低于感染存活患者的99% MFI（p<0.01）和未发生感染患者的78% MFI（p<0.05）。连续样本显示，在7例严重败血症存活患者中有4例出现超血清反应，但在7例死于感染的患者中均未出现。
