硒对双酚 A 诱导的小鼠睾丸线粒体氧化应激及精子运动能力的保护作用。
Protective effects of selenium on Bisphenol A-induced oxidative stress in mouse testicular mitochondria and sperm motility.
机构信息
Student Research committee, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
Department of Anatomical Sciences, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
出版信息
JBRA Assist Reprod. 2021 Jul 21;25(3):459-465. doi: 10.5935/1518-0557.20210010.
OBJECTIVE
This study aimed to explore the impact of selenium (SE) on Bisphenol-A (BPA)-exposed sperm and isolated testicular mitochondria of mice.
METHODS
Mouse sperm and isolated mitochondria were exposed to BPA (0.8 mM) and different concentrations of SE (50, 100, and 200 μM) for four hours. The viability of sperm and isolated mitochondria as well as the mitochondrial membrane potential (MMP) were evaluated. SOD (superoxide dismutase), GSH (glutathione), MDA (malondialdehyde), and ROS (reactive oxygen species) levels in testicular mitochondria were also examined.
RESULTS
BPA concentration-dependently enhanced ROS and MDA levels in isolated mitochondria, while MMP and acclivity of GSH and SOD significantly reduced. BPA also considerably impaired spermatozoa survival and motility. SE concentration-dependently reduced mitochondrial oxidative stress, MMP, sperm survival, and total sperm motility.
CONCLUSIONS
Our findings collectively suggested that SE concentration-dependently reversed BPA-caused mitochondrial toxicity and reduced sperm motility by suppressing oxidative stress.
目的
本研究旨在探讨硒（SE）对双酚 A（BPA）暴露的精子和小鼠离体睾丸线粒体的影响。
方法
将小鼠精子和离体线粒体分别暴露于 BPA（0.8mM）和不同浓度的 SE（50、100 和 200μM）中 4 小时。评估精子和离体线粒体的活力以及线粒体膜电位（MMP）。还检测了睾丸线粒体中超氧化物歧化酶（SOD）、谷胱甘肽（GSH）、丙二醛（MDA）和活性氧（ROS）的水平。
结果
BPA 浓度依赖性地增加了离体线粒体中的 ROS 和 MDA 水平，而 MMP 和 GSH 和 SOD 的活性则显著降低。BPA 还严重损害了精子的存活率和活力。SE 浓度依赖性地降低了线粒体氧化应激、MMP、精子存活率和总精子活力。
结论
我们的研究结果表明，SE 浓度依赖性地通过抑制氧化应激逆转了 BPA 引起的线粒体毒性，并降低了精子的活力。
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