Zhu Yihao, Jiao Xiaoyan, An Yang, Li Shu, Teng Xiaohua
College of Animal Science and Technology, Northeast Agricultural University, Harbin 150030, People's Republic of China.
College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, People's Republic of China.
Oncotarget. 2017 Nov 20;8(64):108130-108145. doi: 10.18632/oncotarget.22553. eCollection 2017 Dec 8.
To investigate alleviative effect of selenium (Se) on lead (Pb)-induced apoptosis in chicken nervous tissues, 7-day-old chickens were randomly divided into four groups. The control group was fed a standard diet and drinking water. In the Pb and Se/Pb groups, (CHOO)Pb was dissolved in drinking water. In the Se and Se/Pb groups, NaSeO was put into the standard diet. Embryonic neurocytes were divided into the control, Se (containing NaSeO), Pb (containing (CHCOO)Pb), and Se/Pb (containing NaSeO and (CHCOO)Pb) groups. The following contents were performed: Morphologic observation for 90 days in brain tissues and for 12, 24, 36, and 48 hours in embryonic neurocytes; and antioxidant indexes, messenger RNA (mRNA) expression of twenty-five selenoproteins, and mRNA and protein expression of five apoptosis-related genes for 30, 60, and 90 days in brain tissues and for 12, 24, 36, and 48 hours in embryonic neurocytes. The results indicated that Se alleviated Pb-caused morphological changes; the decrease of superoxide dismutase, glutathione peroxidase (GPx), GPx1, GPx2, GPx3, GPx4, thioredoxin reductases (Txnrd)1, Txnrd2, Txnrd3, iodothyronine deiodinases (Dio)1, Dio2, Dio3, selenoprotein (Sel)T, SelK, SelS, SelH, SelM, SelU, SelI, SelO, Selpb, selenoprotein (Sep)n1, Sepp1, Sepx1, Sepw1, 15-kDa selenoprotein, and selenophosphate synthetases 2, and B-cell lymphoma-2 (Bcl-2); the increase of malondialdehyde, p53, Bcl-2 associated X protein, cytochrome c, and Caspase-3. Pb had time-dependent effects on GPx4, SelM, and malondialdehyde in the brain tissues; and on SelU in the embryonic neurocytes. Our data demonstrated that Se alleviated Pb-induced apoptosis in the chicken nervous tissues via mitochondrial pathway.
为研究硒（Se）对铅（Pb）诱导的鸡神经组织细胞凋亡的缓解作用，将7日龄雏鸡随机分为四组。对照组给予标准日粮和饮用水。在铅组和硒/铅组中，将醋酸铅溶解于饮用水中。在硒组和硒/铅组中，将亚硒酸钠添加到标准日粮中。将胚胎神经细胞分为对照组、硒组（含亚硒酸钠）、铅组（含醋酸铅）和硒/铅组（含亚硒酸钠和醋酸铅）。进行了以下实验内容：对脑组织进行90天的形态学观察，对胚胎神经细胞进行12、24、36和48小时的形态学观察；并在脑组织中分别于30、60和90天以及在胚胎神经细胞中分别于12、24、36和48小时检测抗氧化指标、25种硒蛋白的信使核糖核酸（mRNA）表达以及5种凋亡相关基因的mRNA和蛋白表达。结果表明，硒减轻了铅引起的形态学变化；减轻了超氧化物歧化酶、谷胱甘肽过氧化物酶（GPx）、GPx1、GPx2、GPx3、GPx4、硫氧还蛋白还原酶（Txnrd）1、Txnrd2、Txnrd3、碘甲状腺原氨酸脱碘酶（Dio）1、Dio2、Dio3、硒蛋白（Sel）T、SelK、SelS、SelH、SelM、SelU、SelI、SelO、Selpb、硒蛋白（Sep）n1、Sepp1、Sepx1、Sepw1、15 kDa硒蛋白和硒磷酸合成酶2以及B细胞淋巴瘤-2（Bcl-2）的降低；减轻了丙二醛、p53、Bcl-2相关X蛋白、细胞色素c和半胱天冬酶-3的升高。铅对脑组织中的GPx4、SelM和丙二醛以及胚胎神经细胞中的SelU具有时间依赖性影响。我们的数据表明，硒通过线粒体途径减轻了铅诱导的鸡神经组织细胞凋亡。
