Chen Wei-Ting, Lin Guan-Bo, Kuo Yu-Yi, Hsieh Chih-Hsiung, Lu Chueh-Hsuan, Sun Yi-Kun, Chao Chih-Yu
Department of Physics, Lab for Medical Physics & Biomedical Engineering, National Taiwan University, Taipei, Taiwan.
Biomedical & Molecular Imaging Center, National Taiwan University College of Medicine, Taipei, Taiwan.
PLoS One. 2021 Apr 26;16(4):e0250491. doi: 10.1371/journal.pone.0250491. eCollection 2021.
As the most common type of neurodegenerative diseases (NDDs), Alzheimer's disease (AD) is thought to be caused mainly by the excessive aggregation of β-amyloid protein (Aβ). However, a growing number of studies have found that reactive oxygen species (ROS) play a key role in the onset and progression of AD. The present study aimed to probe the neuroprotective effect of high-frequency low-intensity pulsed electric field (H-LIPEF) for SH-SY5Y cells against hydrogen peroxide (H2O2) and Aβ-induced cytotoxicity. By looking in a systematic way into the frequency- and amplitude-dependent neuroprotective effect of pulsed electric field (PEF), the study finds that H-LIPEF at 200 Hz produces the optimal protective effect for SH-SY5Y cells. The underlying mechanisms were confirmed to be due to the activation of extracellular signal-regulated kinase (ERK) pathway and the downstream prosurvival and antioxidant proteins. Because the electric field can be modified to focus on specific area in a non-contact manner, the study suggests that H-LIPEF holds great potential for treating NDDs, whose effect can be further augmented with the administering of drugs or natural compounds at the same time.
作为最常见的神经退行性疾病（NDDs）类型，阿尔茨海默病（AD）被认为主要由β-淀粉样蛋白（Aβ）的过度聚集引起。然而，越来越多的研究发现活性氧（ROS）在AD的发病和进展中起关键作用。本研究旨在探究高频低强度脉冲电场（H-LIPEF）对SH-SY5Y细胞抗过氧化氢（H2O2）和Aβ诱导的细胞毒性的神经保护作用。通过系统研究脉冲电场（PEF）的频率和幅度依赖性神经保护作用，该研究发现200 Hz的H-LIPEF对SH-SY5Y细胞产生最佳保护作用。其潜在机制被证实是由于细胞外信号调节激酶（ERK）通路以及下游促生存和抗氧化蛋白的激活。由于电场可以以非接触方式进行调整以聚焦于特定区域，该研究表明H-LIPEF在治疗NDDs方面具有巨大潜力，同时给予药物或天然化合物可进一步增强其效果。
