虾青素和岩藻黄质对淀粉样蛋白 β（Aβ）毒性和聚集的神经保护活性的体外研究。
In vitro studies of the neuroprotective activities of astaxanthin and fucoxanthin against amyloid beta (Aβ) toxicity and aggregation.
机构信息
Centre for Marine Bioproducts Development (CMBD), College of Medicine and Public Health, Flinders University, GPO Box 2100, Adelaide, 5001, South Australia, Australia; Medical Biotechnology, College of Medicine and Public Health, Flinders University, GPO Box 2100, Adelaide, 5001, South Australia, Australia; Ministry of Higher Education in Saudi Arabia, King Faisal Hospital Street, Riyadh, 11153, Saudi Arabia.
Discipline of Pharmacology, School of Medicine, Faculty of Health Sciences, The University of Adelaide, Adelaide, South Australia, Australia.
出版信息
Neurochem Int. 2019 Mar;124:215-224. doi: 10.1016/j.neuint.2019.01.010. Epub 2019 Jan 9.
Amyloid beta (Aβ) can aggregate and form plaques, which are considered as one of the major hallmarks of Alzheimer's disease. This study aims to directly compare the neuroprotective activities in vitro of two marine-derived carotenoids astaxanthin and fucoxanthin that have shown a spectrum of biological activities, including neuroprotection. The in vitro neuroprotective activities were investigated against Aβ-mediated toxicity in pheochromocytoma (PC-12) neuronal cells using the MTT cell viability assay, anti-apoptotic, antioxidant and neurite outgrowth activities; as well as inhibition against Aβ fibrillization in the Thioflavin T (ThT) assay of fibril kinetics and via transmission electron microscopic (TEM) evaluation of fibril morphology. The results demonstrated that both astaxanthin and fucoxanthin exhibited multi-neuroprotective effects favouring fucoxanthin over astaxanthin supporting neuroprotective roles of marine-derived carotenoids as potential novel dementia prevention or therapeutic strategies.
淀粉样蛋白β（Aβ）可以聚集并形成斑块，这被认为是阿尔茨海默病的主要标志之一。本研究旨在直接比较两种海洋衍生类胡萝卜素虾青素和岩藻黄质的体外神经保护活性，这两种物质具有多种生物活性，包括神经保护作用。通过 MTT 细胞活力测定、抗细胞凋亡、抗氧化和神经突生长活性，以及在 Thioflavin T（ThT）测定中抑制 Aβ 纤维形成的纤维动力学和透射电子显微镜（TEM）评估纤维形态，研究了这两种物质对儿茶酚胺（PC-12）神经元细胞中 Aβ 介导的毒性的体外神经保护活性。结果表明，虾青素和岩藻黄质都表现出多种神经保护作用，岩藻黄质的作用优于虾青素，支持海洋衍生类胡萝卜素作为潜在的新型痴呆症预防或治疗策略的神经保护作用。
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