Fedak George, Chi Dawn, Wolfe Danielle, Ouellet Thérèse, Cao Wenguang, Han Fangpu, Xue Allen
Ottawa Research and Development Centre, Agriculture and Agri-Food Canada, Ottawa, ON K1A 0C6, Canada.
State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences No.1, Beijing, China.
Genome. 2021 Nov;64(11):997-1008. doi: 10.1139/gen-2020-0151. Epub 2021 Apr 26.
The diploid form of tall wheatgrass, (Host) D.R. Dewey (2 = 2 = 14, EE genome), has a high level of resistance to fusarium head blight. The symptoms did not spread beyond the inoculated florets following point inoculation. Using a series of E-genome chromosome additions in a bread wheat cultivar Chinese Spring (CS) background, the resistance was found to be localized to the long arm of chromosome 7E. The CS mutant was used to induce recombination between chromosome 7E, present in the 7E(7D) substitution and homoeologous wheat chromosomes. Multivalent chromosome associations were detected in the BC hybrids, confirming the effectiveness of the mutant. Genetic markers specific for chromosome 7E were used to estimate the size of the 7E introgression in the wheat genome. Using single sequence repeat (SSR) markers specific for homoeologous wheat chromosome 7, introgressions were detected on wheat chromosomes 7A, 7B, and 7D. Some of the introgression lines were resistant to fusarium head blight.
高冰草的二倍体形态，（寄主）D.R. 杜威（2n = 2x = 14，EE基因组），对镰刀菌穗腐病具有高度抗性。点接种后，症状未扩散到接种的小花之外。在面包小麦品种中国春（CS）背景下使用一系列E基因组染色体附加系，发现抗性定位于7E染色体的长臂上。利用CS突变体诱导存在于7E(7D)代换系中的7E染色体与小麦同源染色体之间的重组。在回交杂种中检测到多价染色体联会，证实了该突变体的有效性。使用7E染色体特异的遗传标记来估计小麦基因组中7E渐渗片段的大小。利用小麦同源染色体7特异的单序列重复（SSR）标记，在小麦7A、7B和7D染色体上检测到渐渗。一些渐渗系对镰刀菌穗腐病具有抗性。
