Song Rongrong, Cheng Yifan, Wen Mingxing, Song Xinying, Wang Tong, Xia Mengshuang, Sun Haojie, Cheng Menghao, Cui Huimin, Yuan Chunxia, Liu Xiaoxue, Wang Zongkuan, Sun Li, Wang Haiyan, Xiao Jin, Wang Xiue
State Key Laboratory of Crop Genetics and Germplasm Enhancement, Cytogenetics Institute, Nanjing Agricultural University/JCIC-MCP, Nanjing, Jiangsu, 210095, China.
Zhenjiang Institute of Agricultural Science, Jurong, Jiangsu, 212400, China.
Theor Appl Genet. 2023 Mar 10;136(3):36. doi: 10.1007/s00122-023-04278-1.
A new FHB resistance locus FhbRc1 was identified from the R. ciliaris chromosome 7S and transferred into common wheat by developing alien translocation lines. Fusarium head blight (FHB) caused by multiple Fusarium species is a globally destructive disease of common wheat. Exploring and utilization of resources with FHB resistance are the most effective and environmentally beneficial approach for the disease control. Roegneria ciliaris (Trin.) Nevski (2n = 4x = 28, SSYY), a tetraploid wheat wild relative, possesses high resistance to FHB. In the previous study, a complete set of wheat-R. ciliaris disomic addition (DA) lines were evaluated for FHB resistance. DA7S had stable FHB resistance, which was confirmed to be derived from alien chromosome 7S. We tentatively designated the resistant locus as FhbRc1. For better utilization of the resistance in wheat breeding, we developed translocations by inducing chromosome structural aberrations using iron irradiation and the homologous pairing gene mutant ph1b. Totally, 26 plants having various 7S structural aberrations were identified. By marker analysis, a cytological map of 7S was constructed and 7S was dissected into 16 cytological bins. Seven alien chromosome aberration lines, which all had the bin 7S-1 on the long arm of 7S, showed enhanced FHB resistance. Thus, FhbRc1 was mapped to the distal region of 7SL. A homozygous translocation line T4BS·4BL-7SL (NAURC001) was developed. It showed improved FHB resistance, while had no obvious genetic linkage drag for the tested agronomic traits compared with the recurrent parent Alondra's. When transferring the FhbRc1 into three different wheat cultivars, the derived progenies having the translocated chromosome 4BS·4BL-7SL all showed improved FHB resistance. This revealed the potential value of the translocation line in wheat breeding for FHB resistance.
从纤毛鹅观草7S染色体上鉴定出一个新的赤霉病抗性位点FhbRc1，并通过培育异源易位系将其导入普通小麦。由多种镰刀菌引起的赤霉病是普通小麦的一种全球性毁灭性病害。探索和利用抗赤霉病资源是控制该病最有效且对环境有益的方法。纤毛鹅观草（Trin.）Nevski（2n = 4x = 28，SSYY），一种四倍体小麦野生近缘种，对赤霉病具有高抗性。在之前的研究中，对一套完整的小麦 - 纤毛鹅观草二体附加（DA）系进行了赤霉病抗性评估。DA7S具有稳定的赤霉病抗性，已证实其源自外源染色体7S。我们暂定将该抗性位点命名为FhbRc1。为了在小麦育种中更好地利用该抗性，我们通过铁辐射诱导染色体结构畸变和利用同源配对基因突变体ph1b培育了易位系。总共鉴定出26株具有各种7S结构畸变的植株。通过标记分析，构建了7S的细胞学图谱，并将7S划分为16个细胞学区间。七个外源染色体畸变系，其7S长臂上均具有7S - 1区间，表现出增强的赤霉病抗性。因此，FhbRc1被定位到7SL的远端区域。培育出了一个纯合易位系T4BS·4BL - 7SL（NAURC001）。它表现出改良的赤霉病抗性，与轮回亲本阿隆德拉相比，在所测试的农艺性状上没有明显的遗传连锁累赘。当将FhbRc1导入三个不同的小麦品种时，具有易位染色体4BS·4BL - 7SL的衍生后代均表现出改良的赤霉病抗性。这揭示了该易位系在小麦抗赤霉病育种中的潜在价值。
