对抹香鲸肌红蛋白和人血红蛋白中血红素取向无序的1H-核磁共振和圆二色性研究。
1H-n.m.r. and c.d. studies of haem orientational disorder in sperm-whale myoglobin and human haemoglobin.
作者信息
Aojula H S, Wilson M T, Moore G R, Williamson D J
机构信息
Department of Chemistry, University of Essex, Wivenhoe Park, Colchester, U.K.
出版信息
Biochem J. 1988 Mar 15;250(3):853-8. doi: 10.1042/bj2500853.
Abstract
1H-n.m.r. and c.d. studies on sperm-whale myoglobin show that the c.d. signal in the Soret region is inversely and linearly related to the proportion of minor isomer present. An alternative method, 'pH jump', is described for inducing orientational disorder in sperm-whale myoglobin without recourse to reconstitution. 1H-n.m.r. studies on human haemoglobin A indicate little heterogeneity in freshly isolated haemoglobin A, but the effect is enhanced in freeze-dried Sigma haemoglobin A.
摘要
对抹香鲸肌红蛋白的1H-核磁共振和圆二色研究表明，索雷特区域的圆二色信号与存在的次要异构体比例呈反比线性关系。描述了一种替代方法“pH跃变”，用于在不借助重组的情况下诱导抹香鲸肌红蛋白的取向无序。对人血红蛋白A的1H-核磁共振研究表明，新鲜分离的血红蛋白A几乎没有异质性，但在冻干的西格玛血红蛋白A中这种效应增强。
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