School of Pharmaceutical Sciences, Capital Medical University, Beijing 100069, China.
School of Public Health, Capital Medical University, Beijing 100069, China.
Spectrochim Acta A Mol Biomol Spectrosc. 2021 Sep 5;258:119824. doi: 10.1016/j.saa.2021.119824. Epub 2021 Apr 20.
A simple probe BHN based on naphthol and benzothiazole is reported for detecting of arginine (Arg) and lysine (Lys) with high selectivity and sensitivity. The BHN in aqueous solution upon reacting with Arg or Lys induced a visible color change from colorless to yellow. The probe BHN can also be employed for fluorescence turn-on sensing of Arg and Lys with the limits of detection (LOD) of 5.20 × 10 μM and 3.69 × 10 μM, respectively. The naked eye colorimetric and fluorimetric detecting is lack of sensitive to other common amino acids including Gly, Ala, Ser, Pro, Val, Thr, Cys, Leu, Ile, Asn, Asp, Glu, Gln, Met, His, and Phe. The sensing mechanism has been proposed by pH investigation and H NMR spectra.
一种基于萘酚和苯并噻唑的简单探针 BHN 被报道用于高选择性和灵敏度地检测精氨酸（Arg）和赖氨酸（Lys）。BHN 在水溶液中与 Arg 或 Lys 反应后，会引起从无色到黄色的可见颜色变化。探针 BHN 还可用于 Arg 和 Lys 的荧光开启感应，检测限（LOD）分别为 5.20×10 μM 和 3.69×10 μM。肉眼比色和荧光检测对其他常见氨基酸（包括 Gly、Ala、Ser、Pro、Val、Thr、Cys、Leu、Ile、Asn、Asp、Glu、Gln、Met、His 和 Phe）缺乏敏感性。通过 pH 研究和 H NMR 光谱提出了传感机制。
