Nanotherapeutics and Biosensor Section, Chemistry Division, Bhabha Atomic Research Centre, Trombay 400 085 Maharashtra, India.
In Vivo Models and Testing Division, Biomedical Technology Wing, Sree Chitra Tirunal Institute for Medical Sciences & Technology, Trivandrum, Kerala 695012, India.
Biomed Mater. 2021 May 11;16(4). doi: 10.1088/1748-605X/abfbbf.
forming tissue adhesives based on biopolymers offer advantages over conventional sutures and staples in terms of biocompatibility, biodegradability, ease of application and improved patient compliance and comfort. Here, we describe the evaluation ofgelling hydrogel system based on dextran dialdehyde (DDA) obtained by periodate oxidization of dextran and chitosan hydrochloride (CH) as tissue adhesive. The hydrogel was prepared by reacting aldehyde functions in DDA with the amino functions in CH via Schiff's reaction. The gelation reaction was instantaneous and took just 4 s. The DDA-CH hydrogel as tissue adhesive was evaluated on a sheep lung parenchymal injury model and a pig aortic model and was compared with the commercially available tissue sealant, Bioglue®. The DDA-CH glue could completely seal the sheep lung incision site even at inflation with air way pressure of 30 cm of HO with no air leak observed in the incision sites (= 8) in any of the animals. Histological analyses showed mild inflammation after 2 weeks, comparable to Bioglue®. Resorption of test material by giant cells with no adverse effect on lung parenchyma was seen after 3 months. The DDA-CH glue was also very effective in sealing aortic incisions in a pig model (= 4) with no failures and aneurisms. The endoluminal surface of the sealed incision in all cases showed intact apposition with adequate healing across the incision. No tissue necrosis or inflammation of endothelial surface could be seen grossly. Our studies show that the DDA-CH hydrogel could function as an effective sealant for the prevention of air and blood leaks following lung and vascular surgery.
基于生物聚合物的组织粘合剂在生物相容性、可生物降解性、易于应用以及提高患者顺应性和舒适度方面优于传统缝线和吻合钉。在这里，我们描述了基于葡聚糖二醛（DDA）的凝胶水凝胶系统的评估，该系统是通过葡聚糖的过碘酸盐氧化和壳聚糖盐酸盐（CH）获得的。水凝胶是通过 DDA 中的醛基与 CH 中的氨基通过席夫碱反应反应制备的。凝胶反应是瞬时的，只需 4 秒。将 DDA-CH 水凝胶作为组织粘合剂在绵羊肺实质损伤模型和猪主动脉模型上进行了评估，并与市售的组织密封剂 Bioglue®进行了比较。DDA-CH 胶甚至可以在充气至 30 cm H2O 的情况下完全密封绵羊肺切口部位，并且在任何动物的切口部位（= 8）都没有观察到空气泄漏。组织学分析显示，在第 2 周后出现轻度炎症，与 Bioglue®相当。在第 3 个月时，观察到巨细胞吸收测试材料，而对肺实质没有不良影响。DDA-CH 胶在猪模型中也非常有效地密封主动脉切口（= 4），没有失败和动脉瘤。在所有情况下，密封切口的内腔表面均显示出完整的贴合，切口处有足够的愈合。大体上没有看到组织坏死或内皮表面的炎症。我们的研究表明，DDA-CH 水凝胶可以作为一种有效的密封剂，用于预防肺和血管手术后的空气和血液泄漏。
