一种高粘性且天然衍生的密封剂。
A highly adhesive and naturally derived sealant.
作者信息
Assmann Alexander, Vegh Andrea, Ghasemi-Rad Mohammad, Bagherifard Sara, Cheng George, Sani Ehsan Shirzaei, Ruiz-Esparza Guillermo U, Noshadi Iman, Lassaletta Antonio D, Gangadharan Sidhu, Tamayol Ali, Khademhosseini Ali, Annabi Nasim
机构信息
Biomaterials Innovation Research Center, Brigham and Women's Hospital, Harvard Medical School, 65 Landsdowne Street, Cambridge, MA, 02139, USA; Harvard-MIT Division of Health Sciences and Technology, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA; Wyss Institute for Biologically Inspired Engineering, Harvard University, Boston, MA, 02115, USA; Department of Cardiovascular Surgery and Research Group for Experimental Surgery, Heinrich Heine University, Medical Faculty, 40225, Duesseldorf, Germany.
Biomaterials Innovation Research Center, Brigham and Women's Hospital, Harvard Medical School, 65 Landsdowne Street, Cambridge, MA, 02139, USA; Harvard-MIT Division of Health Sciences and Technology, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA; Department of Materials Science and Engineering, University of Toronto, Toronto, Ontario, M5S1A4, Canada.
出版信息
Biomaterials. 2017 Sep;140:115-127. doi: 10.1016/j.biomaterials.2017.06.004. Epub 2017 Jun 6.
Conventional surgical techniques to seal and repair defects in highly stressed elastic tissues are insufficient. Therefore, this study aimed to engineer an inexpensive, highly adhesive, biocompatible, and biodegradable sealant based on a modified and naturally derived biopolymer, gelatin methacryloyl (GelMA). We tuned the degree of gelatin modification, prepolymer concentration, photoinitiator concentration, and crosslinking conditions to optimize the physical properties and adhesion of the photocrosslinked GelMA sealants. Following ASTM standard tests that target wound closure strength, shear resistance, and burst pressure, GelMA sealant was shown to exhibit adhesive properties that were superior to clinically used fibrin- and poly(ethylene glycol)-based glues. Chronic in vivo experiments in small as well as translational large animal models proved GelMA to effectively seal large lung leakages without the need for sutures or staples, presenting improved performance as compared to fibrin glue, poly(ethylene glycol) glue and sutures only. Furthermore, high biocompatibility of GelMA sealant was observed, as evidenced by a low inflammatory host response and fast in vivo degradation while allowing for adequate wound healing at the same time. Combining these results with the low costs, ease of synthesis and application of the material, GelMA sealant is envisioned to be commercialized not only as a sealant to stop air leakages, but also as a biocompatible and biodegradable hydrogel to support lung tissue regeneration.
用于密封和修复高应力弹性组织缺损的传统外科技术并不完善。因此，本研究旨在基于一种改性的天然衍生生物聚合物甲基丙烯酰化明胶（GelMA）设计一种价格低廉、高粘性、生物相容性好且可生物降解的密封剂。我们调整了明胶的改性程度、预聚物浓度、光引发剂浓度和交联条件，以优化光交联GelMA密封剂的物理性能和粘附性。按照针对伤口闭合强度、抗剪切性和爆破压力的ASTM标准测试，GelMA密封剂表现出优于临床使用的基于纤维蛋白和聚乙二醇的胶水的粘附性能。在小型以及转化型大型动物模型中的慢性体内实验证明，GelMA能够有效密封大的肺漏气，无需缝合或钉合，与纤维蛋白胶、聚乙二醇胶水和单纯缝合相比，性能有所提高。此外，观察到GelMA密封剂具有高生物相容性，表现为炎症宿主反应低且体内降解快，同时能实现充分的伤口愈合。将这些结果与该材料的低成本、易于合成和应用相结合，GelMA密封剂不仅有望作为一种阻止空气泄漏的密封剂商业化，还可作为一种生物相容性和可生物降解的水凝胶来支持肺组织再生。
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