Department of Pharmaceutical Analysis, College of Pharmacy, Beihua University, Jilin, China.
Department of Hematology, General Hospital of the Chinese People's Liberation Army, Beijing, China.
J Food Biochem. 2021 Jun;45(6):e13740. doi: 10.1111/jfbc.13740. Epub 2021 Apr 26.
The preventive and therapeutic mechanisms of CDBE on osteoporosis were studied by observing the serum bone-related biochemical indicators, bone trabecular micro-structure and intestinal flora in ovariectomized osteoporosis model mice, in order to provide a scientific theoretical basis for the further study on the effect of CDBE on osteoporosis, and the prevention and treatment of osteoporosis with clinical traditional Chinese medicines. The components in CDBE were detected by UHPLC-MS. A mouse osteoporosis model was established by the bilateral ovariectomy in female ICR mice. The biochemical indicators related to osteoporosis were detected, the right proximal tibia was scanned by Micro-CT, the intestinal microflora in the colon contents were examined, and the changes of microflora were taken as the main target to evaluate the effect of CDBE on the intestinal microflora in the model mice. A total of 16 compounds were obtained by the combined application of UHPLC-MS. CDBE could significantly increase the contents of E2, Ca , CT, HyP, OCN, FOXP3, P1NP and CTX-II, in the model mice. CDBE could significantly improve the trabecular micro-structure, Tb.N, Tb.Sp, SMI and Conn.D. CDBE could make the intestinal flora of osteoporosis model mice tend to healthy mice in species and quantity. CDBE can improve the symptoms of postmenopausal osteoporosis in mice, with a positive effect on the intestinal flora of postmenopausal mice. Its mechanism of regulating the symptoms of osteoporosis may be related to the regulation of bone-related biochemical indicators in the serum of mice. PRACTICAL APPLICATIONS: This research has a positive impact on the development of functional food with anti-osteoporosis in the future.
通过观察去卵巢骨质疏松模型小鼠的血清骨相关生化指标、骨小梁微结构和肠道菌群，研究 CDBE 对骨质疏松的防治机制，为进一步研究 CDBE 对骨质疏松的作用以及临床中药防治骨质疏松提供科学的理论依据。方法：采用 UHPLC-MS 检测 CDBE 的成分。通过双侧卵巢切除术建立雌性 ICR 小鼠骨质疏松模型，检测骨质疏松相关生化指标，对右侧胫骨近端进行 Micro-CT 扫描，检测结肠内容物中的肠道菌群，以菌群变化为主要指标评价 CDBE 对模型小鼠肠道菌群的影响。结果：采用 UHPLC-MS 联合应用，共得到 16 种化合物。CDBE 可显著提高模型小鼠血清中 E2、Ca、CT、HyP、OCN、FOXP3、P1NP 和 CTX-II 的含量。CDBE 可显著改善骨质疏松模型小鼠的骨小梁微结构，增加 Tb.N、Tb.Sp、SMI 和 Conn.D。CDBE 可使骨质疏松模型小鼠的肠道菌群在种类和数量上向健康小鼠方向发展。结论：CDBE 可改善去卵巢小鼠的绝经后骨质疏松症状，对绝经后小鼠的肠道菌群有积极影响。其调节骨质疏松症状的机制可能与调节小鼠血清中骨相关生化指标有关。
