通过固态13C核磁共振从取向的蛋黄卵磷脂中获得的化学位移各向异性。
Chemical shift anisotropies obtained from aligned egg yolk phosphatidylcholine by solid-state 13C nuclear magnetic resonance.
作者信息
Braach-Maksvytis V L, Cornell B A
机构信息
Commonwealth Scientific Industrial Research Organisation, New South Wales, Australia.
出版信息
Biophys J. 1988 May;53(5):839-43. doi: 10.1016/S0006-3495(88)83163-1.
Abstract
Natural abundance solid-state 13C NMR spectra were obtained from orientated egg yolk phosphatidylcholine multilayers in which peaks from the different types of carbon in the lipid were resolved. The residual chemical shift anisotropy of the choline, glycerol, and olefinic carbons, as well as the carbonyl and acyl chain methylene carbons, were estimated. This information provided the basis for a qualitative description of the order and conformation of egg yolk phosphatidylcholine in the L alpha phase. The results suggested the gauche conformation for the C alpha-C beta bond in the choline moiety, a constrained glycerol region, a magic angle orientation for the sn-2 carbonyl, and a preferred orientation close to the bilayer normal for the plane of the sn-1 carbonyl bond and acyl chain C = C bond. The orientations of the carbon nuclei are in accord with the molecular conformation derived from previous 2H, 31P, and 13C NMR studies.
摘要
通过对取向的蛋黄磷脂酰胆碱多层膜进行天然丰度固态13C核磁共振光谱分析，分辨出了脂质中不同类型碳的峰。估算了胆碱、甘油和烯碳以及羰基和酰基链亚甲基碳的剩余化学位移各向异性。这些信息为定性描述Lα相中蛋黄磷脂酰胆碱的有序性和构象提供了基础。结果表明，胆碱部分的Cα-Cβ键呈gauche构象，甘油区域受限，sn-2羰基呈魔角取向，sn-1羰基键和酰基链C = C键平面的优选取向接近双层法线。碳核的取向与先前2H、31P和13C核磁共振研究得出的分子构象一致。
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