[小鼠骨髓细胞悬液在器官培养中形成骨组织]
[Formation of bone tissue by mouse bone marrow cell suspensions in organ culture].
作者信息
Luriia E A, Kuznetsov S A, Genkina E N, Fridenshteĭn A Ia
出版信息
Biull Eksp Biol Med. 1988 Jun;105(6):720-3.
PMID:3390592
Abstract
Adult mouse bone marrow cell suspensions prepared by trypsinization were cultivated in gelatin sponges on millipore filters. When HAWP filters were used, multilayer bone structure was formed. It contained mineralized ground substance, incorporated bone cells and osteoblast layer. With the use of AUFS filters, bone tissue developed not only on the top surface, but also inside the filter.
摘要
通过胰蛋白酶消化制备的成年小鼠骨髓细胞悬液在微孔滤膜上的明胶海绵中培养。当使用HAWP滤膜时，形成了多层骨结构。它包含矿化的基质、掺入的骨细胞和成骨细胞层。使用AUFS滤膜时，骨组织不仅在滤膜的上表面形成，也在滤膜内部形成。
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