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苦梓含笑叶提取物对人白血病癌细胞凋亡特性的影响

The Apoptotic Properties of Leaf Extracts of Simarouba glauca against Human Leukemic Cancer Cells.

机构信息

Jawaharlal Nehru Tropical Botanic Garden and Research, Trivandrum, Kerala, India.

Regional Cancer Centre, Trivandrum, Kerala, India.

出版信息

Asian Pac J Cancer Prev. 2021 Apr 1;22(4):1305-1312. doi: 10.31557/APJCP.2021.22.4.1305.

DOI:10.31557/APJCP.2021.22.4.1305
PMID:33906326
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8325137/
Abstract

BACKGROUND AND OBJECTIVE

Simarouba glauca is a plant belonging to the family of Simaroubaceae. It is a potent source of secondary metabolites. The aim of this study was to evaluate the apoptotic properties of leaf extracts of Simarouba glauca against human leukemic cancer cells.

MATERIALS AND METHODS

Cytotoxicity of Simarouba glauca was assessed in the leaf extract of petroleum ether against leukemic cells by MTT assay. To detect the apoptotic features, fluorescence microscopy analysis was done with dual acridine orange/ethidium bromide fluorescent staining and Hoechst staining. To determine the externalization of phosphatidylserine, annexin v staining was done. Mitochondrial or death receptor activation was confirmed by caspase 3 analysis by flow cytometry.

RESULTS

This study revealed that Simarouba glauca was able to treat leukemia. Among the four extracts, petroleum ether extract showed a higher order of in vitro anticancer activity. The petroleum ether extract strongly inhibited the proliferation of K562 cell lines with IC50 values of 186 µg/ml. Dual acridine orange/ethidium bromide fluorescent staining and Hoechst staining revealed the characteristic features of apoptosis. Annexin V confirmed early and late stage apoptosis. Caspase-3 analysis revealed that cell death was due to mitochondrial or death receptor activation in mitochondrial pathway.

CONCLUSION

These findings suggested that Simarouba glauca leaf extracts inhibited leukemic cells in a time- and dose-dependent manner either through mitochondrial or death receptor activation. The leaf extracts of Simarouba glauca was found to be nontoxic to lymphocytes. It can be concluded that Simarouba glauca is an important source of phytochemicals posing efficacy against leukemic cancer cells.
.

摘要

背景与目的

苦配巴是苦木科的一种植物。它是次生代谢产物的重要来源。本研究旨在评估苦配巴叶提取物对人白血病癌细胞的凋亡特性。

材料与方法

采用 MTT 法评估石油醚提取物对白血病细胞的细胞毒性。通过吖啶橙/溴化乙锭双荧光染色和 Hoechst 染色检测凋亡特征。通过 Annexin V 染色检测磷脂酰丝氨酸的外化。通过流式细胞术分析 caspase 3 确定线粒体或死亡受体的激活。

结果

本研究表明，苦配巴能够治疗白血病。在四种提取物中，石油醚提取物表现出更高的体外抗癌活性。石油醚提取物强烈抑制 K562 细胞系的增殖，IC50 值为 186µg/ml。吖啶橙/溴化乙锭双荧光染色和 Hoechst 染色显示出凋亡的特征。Annexin V 证实了早期和晚期凋亡。Caspase-3 分析表明，细胞死亡是由于线粒体或死亡受体在线粒体途径中的激活。

结论

这些发现表明，苦配巴叶提取物以时间和剂量依赖的方式抑制白血病细胞，无论是通过线粒体还是死亡受体激活。苦配巴叶提取物对淋巴细胞无毒性。可以得出结论，苦配巴是一种重要的植物化学物质来源，对白血病癌细胞具有疗效。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6dc2/8325137/6d0a0b6b45ab/APJCP-22-1305-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6dc2/8325137/4d5e1fa0e22e/APJCP-22-1305-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6dc2/8325137/fc07e0a73d61/APJCP-22-1305-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6dc2/8325137/8a68cbb2d430/APJCP-22-1305-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6dc2/8325137/d5c8797d9b59/APJCP-22-1305-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6dc2/8325137/99b65b5d6c60/APJCP-22-1305-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6dc2/8325137/aee17b96e7a3/APJCP-22-1305-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6dc2/8325137/d11b638e1c3e/APJCP-22-1305-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6dc2/8325137/d46fa9eff6c0/APJCP-22-1305-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6dc2/8325137/6d0a0b6b45ab/APJCP-22-1305-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6dc2/8325137/4d5e1fa0e22e/APJCP-22-1305-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6dc2/8325137/fc07e0a73d61/APJCP-22-1305-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6dc2/8325137/8a68cbb2d430/APJCP-22-1305-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6dc2/8325137/d5c8797d9b59/APJCP-22-1305-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6dc2/8325137/99b65b5d6c60/APJCP-22-1305-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6dc2/8325137/aee17b96e7a3/APJCP-22-1305-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6dc2/8325137/d11b638e1c3e/APJCP-22-1305-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6dc2/8325137/d46fa9eff6c0/APJCP-22-1305-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6dc2/8325137/6d0a0b6b45ab/APJCP-22-1305-g009.jpg

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